Sequential microfluidic droplet processing for rapid DNA extraction
文献类型：期刊论文
| 作者 | Pan, Xiaoyan1,2; Zeng, Shaojiang1,2; Zhang, Qingquan1,2; Lin, Bingcheng1; Qin, Jianhua1 |
| 刊名 | electrophoresis
 |
| 出版日期 | 2011-11-01 |
| 卷号 | 32期号:23页码:3399-3405 |
| 关键词 | DNA extraction Droplet processing Droplet splitting Reagent addition |
| 英文摘要 | this work describes a novel droplet-based microfluidic device, which enables sequential droplet processing for rapid dna extraction. the microdevice consists of a droplet generation unit, two reagent addition units and three droplet splitting units. the loading/washing/elution steps required for dna extraction were carried out by sequential microfluidic droplet processing. the movement of superparamagnetic beads, which were used as extraction supports, was controlled with magnetic field. the microdevice could generate about 100 droplets per min, and it took about 1?min for each droplet to perform the whole extraction process. the extraction efficiency was measured to be 46% for ?-dna, and the extracted dna could be used in subsequent genetic analysis such as pcr, demonstrating the potential of the device for fast dna extraction. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | devices ; plugs ; encapsulation ; system ; size |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000298300900013 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142365]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pan, Xiaoyan,Zeng, Shaojiang,Zhang, Qingquan,et al. Sequential microfluidic droplet processing for rapid DNA extraction[J]. electrophoresis,2011,32(23):3399-3405. |
| APA | Pan, Xiaoyan,Zeng, Shaojiang,Zhang, Qingquan,Lin, Bingcheng,&Qin, Jianhua.(2011).Sequential microfluidic droplet processing for rapid DNA extraction.electrophoresis,32(23),3399-3405. |
| MLA | Pan, Xiaoyan,et al."Sequential microfluidic droplet processing for rapid DNA extraction".electrophoresis 32.23(2011):3399-3405. |
入库方式： OAI收割
来源：大连化学物理研究所
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