Phylogenetic diversity and characterization of 2-haloacid degrading bacteria from the marine sponge Hymeniacidon perlevis
文献类型：期刊论文
| 作者 | Huang, Jianyu1,2; Xin, Yanjuan1; Cao, Xupeng1; Zhang, Wei1,3,4 |
| 刊名 | world journal of microbiology & biotechnology
 |
| 出版日期 | 2011-08-01 |
| 卷号 | 27期号:8页码:1787-1794 |
| 关键词 | Bacteria Marine sponge 2-haloacid dehalogenase Salt tolerance Enantioselectivity |
| 英文摘要 | a total of 139 2-haloacid degrading bacteria strains were isolated from the marine sponge hymeniacidon perlevis using a modified enrichment medium and a ph indicator method. after screening on indicator agar and 2-chloropropionic acid (2-cpa) liquid medium, 11 isolates with high degrading activities were characterized and initially identified. seven of the 11 isolates were able to degrade 2-cpa at 8% salt, and four isolates (deh 66, deh 99, deh125 and deh138) degraded 2-cpa at 15% salt. eight of the 11 isolates utilized all four types of organohalogen compounds used in this study. the deh99 and deh138 isolates exhibited the best enantioselectivity towards (s)-2-chloropropionic acid (s-cpa) and (r)-2-chloropropionic acid (r-cpa), respectively. the dehalogenase activities of deh84 against racemic cpa, deh99 against s-cpa, deh138 against r-cpa and deh130 against racemic cpa were 0.16u/mg, 0.06u/mg, 0.12u/mg and 0.19u/mg, respectively. based on 16s rrna sequence analysis, the 11 isolates were clustered into the rhodobacteraceae family of alpha-proteobacteria and the pseudomonadaceae family of gamma-proteobacteria. to our knowledge, this is the first report detailing the isolation of organisms of pseudomonas stuzeri sp. and the rhodobacteraceae family with 2-haloacid dehalogenase activity from marine sponges. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | dl-2-haloacid dehalogenase ; microbial-degradation ; haloacid dehalogenase ; pseudomonas sp ; gene ; purification ; microorganisms ; biochemistry ; sequence ; protein |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000292984000006 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142390]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China 2.Dalian Univ Technol, Minist Educ, Key Lab Ind Ecol & Environm Engn, Sch Environm Sci & Technol, Dalian 116024, Peoples R China 3.Flinders Univ S Australia, Sch Med, Adelaide, SA 5042, Australia 4.Flinders Univ S Australia, Flinders Ctr Marine Bioproc & Bioprod FCMB2, Adelaide, SA 5042, Australia |
| 推荐引用方式 GB/T 7714 | Huang, Jianyu,Xin, Yanjuan,Cao, Xupeng,et al. Phylogenetic diversity and characterization of 2-haloacid degrading bacteria from the marine sponge Hymeniacidon perlevis[J]. world journal of microbiology & biotechnology,2011,27(8):1787-1794. |
| APA | Huang, Jianyu,Xin, Yanjuan,Cao, Xupeng,&Zhang, Wei.(2011).Phylogenetic diversity and characterization of 2-haloacid degrading bacteria from the marine sponge Hymeniacidon perlevis.world journal of microbiology & biotechnology,27(8),1787-1794. |
| MLA | Huang, Jianyu,et al."Phylogenetic diversity and characterization of 2-haloacid degrading bacteria from the marine sponge Hymeniacidon perlevis".world journal of microbiology & biotechnology 27.8(2011):1787-1794. |
入库方式： OAI收割
来源：大连化学物理研究所
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