Hyper-production of C-13-labeled trans-resveratrol in Vitis vinifera suspension cell culture by elicitation and in situ adsorption
文献类型：期刊论文
| 作者 | Yue, Xiangguo1,2; Zhang, Wei1,3,4; Deng, Maicun2 |
| 刊名 | biochemical engineering journal
 |
| 出版日期 | 2011-02-15 |
| 卷号 | 53期号:3页码:292-296 |
| 关键词 | Vitis vinifera C-13-labeled trans-resveratrol Secondary metabolites Plant cell tissue Culture engineering Metabolic control Adsorption |
| 英文摘要 | bioproduction of c-13-labeled trans-resveratrol in plant cell culture has attracted considerable attention with regard to potential applications for human benefit and to better understanding their absorption and in vivo metabolism in humans and animals. in the present work, two elicitors (sa and ja) and adsorbents (hp2mgl) were introduced into the vitis vinifera cell suspension culture, and the results indicated that they could work synergistically to improve the production of trans-resveratrol (2666.7 mg l-1). afterward, 1 mm [1-c-13]-l-phenylalanine (phe) was added to bioproduce c-13-labeled trans-resveratrol with the c-13 enrichment from 35.7% at day 5 to 20.8% at day 10. purification of the products by several chromatographic steps was reported, and the c-13 labeling position was verified using c-13 nmr. our results indicated that, in this case, the rate limiting step of production was the post-biosynthetic events rather than the biosynthesis in elicited culture. furthermore, our results suggested the importance of simultaneous optimization of biosynthetic pathways of metabolites and their post-biosynthetic steps toward achieving commercial plant cell culture. with the help of this technique, we could expect to do industrial scaling-up in a bioreactor to produce high amounts of the non-labeled and c-13-labeled trans-resveratrol in the near future. (c) 2011 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; technology |
| 类目[WOS] | biotechnology & applied microbiology ; engineering, chemical |
| 研究领域[WOS] | biotechnology & applied microbiology ; engineering |
| 关键词[WOS] | differential regulation ; life-span ; synthase ; restriction ; polyphenols ; expression ; cancer ; genes ; wine |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000287625000008 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142411]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Flinders Univ S Australia, Flinders Ctr Marine Bioproc & Bioprod, Adelaide, SA 5042, Australia 4.Flinders Univ S Australia, Dept Med Biotechnol, Sch Med, Adelaide, SA 5042, Australia |
| 推荐引用方式 GB/T 7714 | Yue, Xiangguo,Zhang, Wei,Deng, Maicun. Hyper-production of C-13-labeled trans-resveratrol in Vitis vinifera suspension cell culture by elicitation and in situ adsorption[J]. biochemical engineering journal,2011,53(3):292-296. |
| APA | Yue, Xiangguo,Zhang, Wei,&Deng, Maicun.(2011).Hyper-production of C-13-labeled trans-resveratrol in Vitis vinifera suspension cell culture by elicitation and in situ adsorption.biochemical engineering journal,53(3),292-296. |
| MLA | Yue, Xiangguo,et al."Hyper-production of C-13-labeled trans-resveratrol in Vitis vinifera suspension cell culture by elicitation and in situ adsorption".biochemical engineering journal 53.3(2011):292-296. |
入库方式： OAI收割
来源：大连化学物理研究所
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