Isolation, characterization and identification of a Paracoccus sp 2-haloacid-degrading bacterium from the marine sponge Hymeniacidon perlevis
文献类型：期刊论文
| 作者 | Huang, Jianyu1,2; Xin, Yanjuan1,2; Zhang, Wei1,3,4 |
| 刊名 | journal of basic microbiology
 |
| 出版日期 | 2011-06-01 |
| 卷号 | 51期号:3页码:318-324 |
| 关键词 | 2-haloacid dehalogenase marine sponge stereoselectivity Hymeniacidon perlevis |
| 英文摘要 | a 2-haloacid dehalogenase-producing bacterium, designated deh99, was isolated from the marine sponge hymeniacidon perlevis using a modified enrichment medium and a ph indicator method. deh99 could degrade only half of the racemic mixture 2-chloropropionic acid (2-cpa) in the medium. the dehalogenase of deh99 was further determined to be a (s)-2-haloacid dehalogenase, which can degrade 2-cpa, 2-bromopropionic acid (2-bpa), and iodoacetic acid. the gene encoding the (s)-2-haloacid dehalogenase was partially sequenced and classified into the group ii family. the 2-haloacid dehalogenase showed the highest sequence similarity (77% with 21% query coverage) to the haloacid dehalogenase (dhlb) gene of xanthobacter autotrophicus. a phylogenetic analysis of the 16s rdna sequence demonstrated that the isolate deh99 is a member of the genus paracoccus. to our knowledge, this is the first report detailing the isolation of a strain of genus paracoccus having 2-haloacid dehalogenase activity from marine sponges. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | microbiology |
| 研究领域[WOS] | microbiology |
| 关键词[WOS] | acid dehalogenase ; haloacid dehalogenase ; pseudomonas-putida ; gene ; purification ; sequence ; cloning |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000291107500011 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142430]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Flinders Univ S Australia, Flinders Ctr Marine Bioproc & Bioprod, Sch Med, Adelaide, SA, Australia 4.Flinders Univ S Australia, Dept Med Biotechnol, Sch Med, Adelaide, SA, Australia |
| 推荐引用方式 GB/T 7714 | Huang, Jianyu,Xin, Yanjuan,Zhang, Wei. Isolation, characterization and identification of a Paracoccus sp 2-haloacid-degrading bacterium from the marine sponge Hymeniacidon perlevis[J]. journal of basic microbiology,2011,51(3):318-324. |
| APA | Huang, Jianyu,Xin, Yanjuan,&Zhang, Wei.(2011).Isolation, characterization and identification of a Paracoccus sp 2-haloacid-degrading bacterium from the marine sponge Hymeniacidon perlevis.journal of basic microbiology,51(3),318-324. |
| MLA | Huang, Jianyu,et al."Isolation, characterization and identification of a Paracoccus sp 2-haloacid-degrading bacterium from the marine sponge Hymeniacidon perlevis".journal of basic microbiology 51.3(2011):318-324. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。