Proteomic reactors and their applications in biology
文献类型：期刊论文
| 作者 | Zhou, Hu1,2,3; Ning, Zhibin1,3; Wang, Fangjun1,3,4; Seebun, Deeptee1,3; Figeys, Daniel1,3 |
| 刊名 | febs journal
 |
| 出版日期 | 2011-10-01 |
| 卷号 | 278期号:20页码:3796-3806 |
| 关键词 | fractionation immobilized enzyme reactor liquid chromatography mass spectrometry microfluidic post-translational modification proteomic reactor proteomics strong anion exchange strong cation exchange |
| 英文摘要 | proteomic analysis requires the combination of an extensive suite of technologies including protein processing and separation, micro-flow hplc, ms and bioinformatics. although proteomic technologies are still in flux, approaches that bypass gel electrophoresis (gel-free approaches) are dominating the field of proteomics. along with the development of gel-free proteomics, came the development of devices for the processing of proteomic samples termed proteomic reactors. these microfluidic devices provide rapid, robust and efficient pre-ms sample procession by performing protein sample preparation/concentration, digestion and peptide fractionation. the proteomic reactor has advanced in two major directions: immobilized enzyme reactor and ion exchange-based proteomic reactor. this review summarizes the technical developments and biological applications of the proteomic reactor over the last decade. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | strong cation-exchange ; highly efficient proteolysis ; tandem mass-spectrometry ; protein identification ; liquid-chromatography ; electrospray-ionization ; microfluidic reactor ; peptide separation ; membrane proteome ; n-glycoproteome |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000295335500002 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142505]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Univ Ottawa, Dept Biochem Microbiol & Immunol, Ottawa, ON K1H 8M5, Canada 2.Chinese Acad Sci, Shanghai Inst Mat Med, Beijing 100864, Peoples R China 3.Univ Ottawa, OISB, Ottawa, ON K1H 8M5, Canada 4.Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Hu,Ning, Zhibin,Wang, Fangjun,et al. Proteomic reactors and their applications in biology[J]. febs journal,2011,278(20):3796-3806. |
| APA | Zhou, Hu,Ning, Zhibin,Wang, Fangjun,Seebun, Deeptee,&Figeys, Daniel.(2011).Proteomic reactors and their applications in biology.febs journal,278(20),3796-3806. |
| MLA | Zhou, Hu,et al."Proteomic reactors and their applications in biology".febs journal 278.20(2011):3796-3806. |
入库方式： OAI收割
来源：大连化学物理研究所
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