Wnt/beta-Catenin Signaling Down-regulates N-Acetylglucosaminyltransferase III Expression THE IMPLICATIONS OF TWO MUTUALLY EXCLUSIVE PATHWAYS FOR REGULATION
文献类型：期刊论文
| 作者 | Xu, Qingsong1,2; Akama, Ryota2; Isaji, Tomoya2; Lu, Yingying2; Hashimoto, Hirokazu2; Kariya, Yoshinobu2; Fukuda, Tomohiko2; Du, Yuguang1; Gu, Jianguo2 |
| 刊名 | journal of biological chemistry
 |
| 出版日期 | 2011-02-01 |
| 卷号 | 286期号:6页码:4310-4318 |
| 英文摘要 | in previous studies, we reported that n-acetylglucosaminyl-transferase iii (gnt-iii) activity and the enzyme product, bisected n-glycans, both were induced in cells cultured under dense conditions in an e-cadherin-dependent manner (iijima, j., zhao, y., isaji, t., kameyama, a., nakaya, s., wang, x., ihara, h., cheng, x., nakagawa, t., miyoshi, e., kondo, a., narimatsu, h., taniguchi, n., and gu, j. (2006) j. biol. chem. 281, 13038-13046). furthermore, we found that alpha-catenin, a component of the e-cadherin-catenin complex, was also required for this induction (akama, r., sato, y., kariya, y., isaji, t., fukuda, t., lu, l., taniguchi, n., ozawa, m., and gu, j. (2008) proteomics 8, 3221-3228). to further explore the molecular mechanism of this regulation, the roles of beta-catenin, an essential molecule in both cadherin-mediated cell adhesion and canonical wnt signaling, were investigated. unexpectedly, shrna knockdown of beta-catenin resulted in a dramatic increase in gnt-iii expression and its product, the bisected n-glycans, which was confirmed by rt-pcr and gnt-iii activity and by e4-pha lectin blot analysis. the induction of gnt-iii expression increased bisecting glcnac residues on beta 1 integrin, which led to down-regulation of integrin-mediated cell adhesion and cell migration. immunostaining showed that nuclear localization of beta-catenin was greatly suppressed; intriguingly, the knockdown of beta-catenin in the nuclei was more effective than that in cell-cell contacts in the knockdown cells, which was also confirmed by western blot analysis. stimulation of the wnt signaling pathway by the addition of exogenous wnt3a or bio, a gsk-3 beta inhibitor, consistently and significantly inhibited gnt-iii expression and its products. conversely, the inhibition of beta-catenin translocation into the nuclei increased gnt-iii activation. taken together, the results of the present study are the first to clearly demonstrate that gnt-iii expression may be precisely regulated by the interplay of e-cadherin-catenin complex-mediated cell-cell adhesion and wnt/beta-catenin signaling, which are both crucial in the process of epithelial-mesenchymal transitions in physiological and pathological conditions. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | mediated cell-adhesion ; beta-propeller domain ; e-cadherin ; alpha-catenin ; endoplasmic-reticulum ; protein glycosylation ; breast-cancer ; complex ; disease ; metastasis |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000286975700030 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142525]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Tohoku Pharmaceut Univ, Div Regulatory Glycobiol, Inst Mol Biomembrane & Glycobiol, Aoba Ku, Sendai, Miyagi 9818558, Japan |
| 推荐引用方式 GB/T 7714 | Xu, Qingsong,Akama, Ryota,Isaji, Tomoya,et al. Wnt/beta-Catenin Signaling Down-regulates N-Acetylglucosaminyltransferase III Expression THE IMPLICATIONS OF TWO MUTUALLY EXCLUSIVE PATHWAYS FOR REGULATION[J]. journal of biological chemistry,2011,286(6):4310-4318. |
| APA | Xu, Qingsong.,Akama, Ryota.,Isaji, Tomoya.,Lu, Yingying.,Hashimoto, Hirokazu.,...&Gu, Jianguo.(2011).Wnt/beta-Catenin Signaling Down-regulates N-Acetylglucosaminyltransferase III Expression THE IMPLICATIONS OF TWO MUTUALLY EXCLUSIVE PATHWAYS FOR REGULATION.journal of biological chemistry,286(6),4310-4318. |
| MLA | Xu, Qingsong,et al."Wnt/beta-Catenin Signaling Down-regulates N-Acetylglucosaminyltransferase III Expression THE IMPLICATIONS OF TWO MUTUALLY EXCLUSIVE PATHWAYS FOR REGULATION".journal of biological chemistry 286.6(2011):4310-4318. |
入库方式： OAI收割
来源：大连化学物理研究所
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