Ionic liquid-assisted SDS-PAGE to improve human serum protein separation
文献类型：期刊论文
| 作者 | Zhang, Tao1; Gai, Qingqing1; Qu, Feng1; Zhang, Yukui2 |
| 刊名 | electrophoresis
 |
| 出版日期 | 2011-10-01 |
| 卷号 | 32期号:20页码:2904-2910 |
| 关键词 | Human serum ILs-SDS-PAGE Ionic liquids Protein separation |
| 英文摘要 | ionic liquid (il)-assisted sodium dodecyl sulfate polyacrylamide gel electrophoresis (ils-sds-page) was presented to improve protein separation. ils were employed during the preparation process of polyacrylamide gel, then the modified gel was used for commercial protein marker, binary bovine serum albumin/lysozyme (bsa/lyz) and human serum separation. the influence of ionic liquid concentration, cation alkyl chain length, cation and anion types on proteins separation were investigated. the results showed that ils played a role in improving some protein separation, and ils-sds-page provided higher resolution and separation efficiency than ordinary sds-page for low and middle relative molecular mass proteins in human serum. in addition, the principle of ils-sds-page was discussed and the comparison of ils-sds-page with ordinary sds-page and native page was made. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | polyacrylamide-gel electrophoresis ; blue native electrophoresis ; capillary-electrophoresis ; molecular-weight ; mass-spectrometry ; basic-proteins ; 2-d page ; proteomics ; chromatography ; electrolyte |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000297424500018 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142541]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Tao,Gai, Qingqing,Qu, Feng,et al. Ionic liquid-assisted SDS-PAGE to improve human serum protein separation[J]. electrophoresis,2011,32(20):2904-2910. |
| APA | Zhang, Tao,Gai, Qingqing,Qu, Feng,&Zhang, Yukui.(2011).Ionic liquid-assisted SDS-PAGE to improve human serum protein separation.electrophoresis,32(20),2904-2910. |
| MLA | Zhang, Tao,et al."Ionic liquid-assisted SDS-PAGE to improve human serum protein separation".electrophoresis 32.20(2011):2904-2910. |
入库方式： OAI收割
来源：大连化学物理研究所
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