Purification and characterization of a beta-1,3-glucomannanase expressed in Pichia pastoris
文献类型：期刊论文
| 作者 | Yang, Fan1,3; Zhang, Sufang1; Jin, Guojie1,3; Lin, Xinping1,3; Zhao, Zongbao K.1,2 |
| 刊名 | enzyme and microbial technology
 |
| 出版日期 | 2011-07-10 |
| 卷号 | 49期号:2页码:223-228 |
| 关键词 | beta-1 Secretory expression Pichia pastoris Cell wall Enzyme activity 3-glucomannanase |
| 英文摘要 | the glycoside hydrolase beta-1,3-glucomannanase is an enzyme that specifically breaks the beta-1,3 glycosidic bond of the glucomannan, the main cell wall constituent of some yeasts. in this work, a codon optimized dna sequence of the man5c gene from penicillium iilacinum atcc 36010 was expressed in the yeast pichia pastoris under the control of aox1 promoter. the recombinant protein piman5c was purified from the shake flask culture and the stirred-tank bioreactor culture in yields of 30.0 mg/l and 224.0 mg/l, respectively. the purified protein had a specific activity of 14.6 u/mg at 37 degrees c, ph 4.5. biochemical analysis showed that the optimal temperature and ph for piman5c were 50 degrees c and 4.5, respectively. the recombinant piman5c was efficient in lysis of the cell wall of the red yeast rhodosporidium toruloides to form protoplast. our work provided an effective system for heterogeneous production of beta-1,3-glucomannanase, which should facilitate a more convenient application of this enzyme in biotechnology and other related areas. (c) 2011 elsevier inc. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | yeast-cell wall ; lytic enzyme ; rhodosporidium-toruloides ; saccharomyces-cerevisiae ; schizophyllum-commune ; protoplast formation ; gene ; identification |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000293439100017 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142544]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.CAS, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China 2.Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Fan,Zhang, Sufang,Jin, Guojie,et al. Purification and characterization of a beta-1,3-glucomannanase expressed in Pichia pastoris[J]. enzyme and microbial technology,2011,49(2):223-228. |
| APA | Yang, Fan,Zhang, Sufang,Jin, Guojie,Lin, Xinping,&Zhao, Zongbao K..(2011).Purification and characterization of a beta-1,3-glucomannanase expressed in Pichia pastoris.enzyme and microbial technology,49(2),223-228. |
| MLA | Yang, Fan,et al."Purification and characterization of a beta-1,3-glucomannanase expressed in Pichia pastoris".enzyme and microbial technology 49.2(2011):223-228. |
入库方式： OAI收割
来源：大连化学物理研究所
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