Octyl-functionalized hybrid magnetic mesoporous microspheres for enrichment of low-concentration peptides prior to direct analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
文献类型：期刊论文
| 作者 | Sun, Liangliang1,2; Zhao, Qun1,2; Zhu, Guijie1; Zhou, Yuan1,2; Wang, Tingting1,2; Shan, Yichu1; Yang, Kaiguang1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1 |
| 刊名 | rapid communications in mass spectrometry
 |
| 出版日期 | 2011-05-15 |
| 卷号 | 25期号:9页码:1257-1265 |
| 英文摘要 | octyl-functionalized hybrid magnetic mesoporous (fe(3)o(4)center dot nsio(2)center dot meso-hybrid-c8) microspheres were synthesized and applied in the isolation and pre-concentration of low-concentration peptides prior to direct analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms). such microspheres possess high surface area (324 m(2)/g), hydrophobic group (c8), relatively large pore volume (0.304 cm(3)/g), uniform pore diameter (similar to 3.7 nm), and magnetic responsivity, which make them a simple and efficient kind of adsorbent for the enrichment of low-concentration peptides. for bovine serum albumin (bsa, 15 fmol mu l(-1)) digest, after concentration by fe(3)o(4)center dot nsio(2)center dot meso-hybrid-c8 microspheres, the enrichment performance was evidently better than those obtained by solvent evaporation and c8-functionalized magnetic particles, and comparable to those obtained by commercial anchor chip target and ziptipc18 pipette tip. such microspheres were further applied in the enrichment of the tryptic digests of rat cerebellum proteins and endogenous peptides of crude human serum, and more peaks with higher signal-to-noise (s/n) ratio were obtained than before pre-concentration. furthermore, the pre-concentration reproducibility of magnetic microspheres for biological samples was good, and the limit of detection (lod) for bsa digests by maldi-tof ms was decreased by at least one order of magnitude compared with that obtained without pre-concentration. all the above-mentioned results indicate that the synthesized fe(3)o(4)center dot nsio(2)center dot meso-hybrid-c8 microspheres are promising for the enrichment of low-concentration peptides from complex biosamples. copyright (c) 2011 john wiley & sons, ltd. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences ; technology |
| 类目[WOS] | biochemical research methods ; chemistry, analytical ; spectroscopy |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry ; spectroscopy |
| 关键词[WOS] | tof-ms analysis ; low-abundance peptides ; capillary electrochromatography ; highly efficient ; monolithic column ; silica particles ; human plasma ; sio2 shell ; proteins ; nanoparticles |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000290452600017 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142573]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Liangliang,Zhao, Qun,Zhu, Guijie,et al. Octyl-functionalized hybrid magnetic mesoporous microspheres for enrichment of low-concentration peptides prior to direct analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry[J]. rapid communications in mass spectrometry,2011,25(9):1257-1265. |
| APA | Sun, Liangliang.,Zhao, Qun.,Zhu, Guijie.,Zhou, Yuan.,Wang, Tingting.,...&Zhang, Yukui.(2011).Octyl-functionalized hybrid magnetic mesoporous microspheres for enrichment of low-concentration peptides prior to direct analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.rapid communications in mass spectrometry,25(9),1257-1265. |
| MLA | Sun, Liangliang,et al."Octyl-functionalized hybrid magnetic mesoporous microspheres for enrichment of low-concentration peptides prior to direct analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry".rapid communications in mass spectrometry 25.9(2011):1257-1265. |
入库方式： OAI收割
来源：大连化学物理研究所
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