Preparation and characterization of uniformly sized molecularly imprinted polymers functionalized with core-shell magnetic nanoparticles for the recognition and enrichment of protein
文献类型：期刊论文
| 作者 | Gao, Ruixia1; Kong, Xuan1; Wang, Xin1; He, Xiwen1; Chen, Langxing1; Zhang, Yukui1,2 |
| 刊名 | journal of materials chemistry
 |
| 出版日期 | 2011 |
| 卷号 | 21期号:44页码:17863-17871 |
| 英文摘要 | a general method to prepare thin, molecularly imprinted polymer (mip) coatings on magnetic fe(3)o(4) nanoparticles (nps) with a uniform core-shell structure for the recognition and enrichment of protein was developed. four proteins (bovine serum albumin (bsa, pi = 4.9), bovine hemoglobin (bhb, pi = 6.9), bovine pancreas ribonuclease a (rnase a, pi = 9.4) and lysozyme (lyz, pi = 11.2)) with different isoelectric points were chosen as the templates. the magnetic protein-mips were synthesized by combining surface imprinting and sol-gel techniques. the morphology, adsorption and recognition properties of the magnetic molecularly imprinted nps were investigated by transmission electron microscopy (tem), scanning electron microscopy (sem), x-ray diffraction (xrd), and fourier transform infrared (ft-ir) spectroscopy and through the use of a vibrating sample magnetometer (vsm). in comparison with the use of lyz, bsa and rnase a as template proteins, bhb-imprinted fe(3)o(4) showed the best imprinting effect and the highest adsorption capacity among the four proteins. the as-prepared fe(3)o(4)@ bhb-mips nps with a mean diameter of 230 nm were coated with an mip shell that was 10 nm thick, which enabled the fe(3)o(4)@bhb-mips to easily reach adsorption equilibrium. a high magnetic saturation value of 25.47 emu g(-1) for fe(3)o(4)@bhb-mips nps was obtained, which endowed the adsorbent with the convenience of magnetic separation under an external magnetic field. the resultant fe(3)o(4)@bhb-mips nps could not only selectively extract a target protein from mixed proteins but also specifically capture the protein bhb from a real sample of bovine blood. in addition, different batches of magnetic mips showed good reproducibility and reusability for at least six repeated cycles. |
| WOS标题词 | science & technology ; physical sciences ; technology |
| 类目[WOS] | chemistry, physical ; materials science, multidisciplinary |
| 研究领域[WOS] | chemistry ; materials science |
| 关键词[WOS] | tof-ms analysis ; iron-oxide nanoparticles ; low-concentration peptides ; histidine-tagged proteins ; bovine hemoglobin ; facile synthesis ; biomedical applications ; silica microspheres ; highly efficient ; particles |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000296390600047 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142613]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Ruixia,Kong, Xuan,Wang, Xin,et al. Preparation and characterization of uniformly sized molecularly imprinted polymers functionalized with core-shell magnetic nanoparticles for the recognition and enrichment of protein[J]. journal of materials chemistry,2011,21(44):17863-17871. |
| APA | Gao, Ruixia,Kong, Xuan,Wang, Xin,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2011).Preparation and characterization of uniformly sized molecularly imprinted polymers functionalized with core-shell magnetic nanoparticles for the recognition and enrichment of protein.journal of materials chemistry,21(44),17863-17871. |
| MLA | Gao, Ruixia,et al."Preparation and characterization of uniformly sized molecularly imprinted polymers functionalized with core-shell magnetic nanoparticles for the recognition and enrichment of protein".journal of materials chemistry 21.44(2011):17863-17871. |
入库方式： OAI收割
来源：大连化学物理研究所
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