High-performance purification of quaternary alkaloids from Corydalis yanhusuo W. T. Wang using a new polar-copolymerized stationary phase
文献类型：期刊论文
| 作者 | Wang, Chaoran; Guo, Zhimou; Zhang, Jing; Zeng, Jing; Zhang, Xiuli; Liang, Xinmiao |
| 刊名 | journal of separation science
 |
| 出版日期 | 2011 |
| 卷号 | 34期号:1页码:53-58 |
| 关键词 | Alkaloids Corydalis yanhusuo W. T. Wang HPLC Polar-copolymerized Purification |
| 英文摘要 | an efficient method for purification of alkaloids from corydalis yanhusuo w. t. wang using hplc was developed, featuring a polar-copolymerized stationary phase named c18hce. as ionizable solutes, the crude alkaloid sample often suffered from serious peak tailing problem on conventional rp-lc columns, and the separation would rapidly became destroyed with the increasing of load amount. however, on the new stationary phase, good peak shapes (asymmetry factor <1.5) as well as good loadability were easily obtained in a commonly used acidic mobile phase condition. the loading amount could reach 10 mg per injection on an analytical c18hce column for laboratory-scale purification. about 6.8 mg of palmatine (hplc purity >98%) and 44.4 mg of dehydrocorydaline (h plc purity >98%) were rapidly derived from 200 mg of the crude alkaloid sample, and the recoveries of these two compounds were 76.5 and 81.7%, respectively. the purified alkaloids were characterized by comparing retention times with standard compounds as well as (1)h-nmr data. the new method is simple and high yielding, and it may provide a promising tool for purification of alkaloids as well as other alkaline compounds. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | liquid-chromatography ; basic compounds ; ph ; separations ; retention ; saxicola ; columns ; bases ; hplc |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000286452700008 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142619]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Key Lab Nat Med, Dalian 116023, Liaoning Prov, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Chaoran,Guo, Zhimou,Zhang, Jing,et al. High-performance purification of quaternary alkaloids from Corydalis yanhusuo W. T. Wang using a new polar-copolymerized stationary phase[J]. journal of separation science,2011,34(1):53-58. |
| APA | Wang, Chaoran,Guo, Zhimou,Zhang, Jing,Zeng, Jing,Zhang, Xiuli,&Liang, Xinmiao.(2011).High-performance purification of quaternary alkaloids from Corydalis yanhusuo W. T. Wang using a new polar-copolymerized stationary phase.journal of separation science,34(1),53-58. |
| MLA | Wang, Chaoran,et al."High-performance purification of quaternary alkaloids from Corydalis yanhusuo W. T. Wang using a new polar-copolymerized stationary phase".journal of separation science 34.1(2011):53-58. |
入库方式： OAI收割
来源：大连化学物理研究所
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