Thermally responsive polymer as a sieving matrix of proteins in capillary gel electrophoresis
文献类型：期刊论文
| 作者 | Yang, Chun1,2; Chang, Chunyan1; Wang, Jun1; Qu, Qishu1; Hu, Xiaoya1; Wang, Yang1 |
| 刊名 | analytical methods
 |
| 出版日期 | 2011-12-01 |
| 卷号 | 3期号:12页码:2717-2720 |
| 英文摘要 | a thermally responsive polymer, termed as p(ipaam-am-bisam), was prepared by the copolymerization of n-isopropyl acrylamide, acrylamide, and n,n'-methylenebisacrylamide. at room temperature this polymer was dissolved in water to form a low viscous solution so that it could be loaded in a capillary easily. when a high voltage was applied on the capillary, the polymer was stimulated by the joule heat and transferred from a liquid state into a solid state, acting as a sieving matrix for the separation of proteins according to their molecular weights. the logarithm values of the molecular weights of the proteins correlated linearly with the migration times. this capillary gel electrophoresis of proteins was carried out using a conventional instrument. using such a copolymer, we can easily realize high-speed, high-efficiency and reproducible capillary gel electrophoresis for proteins without any instrumental rebuilding or investment. |
| WOS标题词 | science & technology ; physical sciences ; life sciences & biomedicine ; technology |
| 类目[WOS] | chemistry, analytical ; food science & technology ; spectroscopy |
| 研究领域[WOS] | chemistry ; food science & technology ; spectroscopy |
| 关键词[WOS] | aqueous chromatography ; stationary phases ; separation ; beads ; system ; dna |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000297562100002 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142665]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Yangzhou Univ, Coll Chem & Chem Engn, Yangzhou 225002, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Chun,Chang, Chunyan,Wang, Jun,et al. Thermally responsive polymer as a sieving matrix of proteins in capillary gel electrophoresis[J]. analytical methods,2011,3(12):2717-2720. |
| APA | Yang, Chun,Chang, Chunyan,Wang, Jun,Qu, Qishu,Hu, Xiaoya,&Wang, Yang.(2011).Thermally responsive polymer as a sieving matrix of proteins in capillary gel electrophoresis.analytical methods,3(12),2717-2720. |
| MLA | Yang, Chun,et al."Thermally responsive polymer as a sieving matrix of proteins in capillary gel electrophoresis".analytical methods 3.12(2011):2717-2720. |
入库方式： OAI收割
来源：大连化学物理研究所
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