Fast preparation of monolithic immobilized pH gradient column by photopolymerization and photografting techniques for isoelectric focusing separation of proteins
文献类型：期刊论文
| 作者 | Liang, Yu1,2; Zhu, Guijie1,2; Wang, Tingting1,2; Zhang, Xiaodan1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1 |
| 刊名 | electrophoresis
 |
| 出版日期 | 2011-10-01 |
| 卷号 | 32期号:20页码:2911-2914 |
| 关键词 | Isoelectric focusing separation Monolithic immobilized pH gradient column Photografting Proteins |
| 英文摘要 | a new method was developed to prepare monolithic immobilized ph gradient (m-ipg) columns in uv-transparent fused-silica capillaries by the 5-min photopolymerization of acrylamide and n,n'-methylenebisacrylamide, followed by the 20-min photografting of the focused ampholine-derived glycidylmethacrylate monomer on the monolithic matrix, by which the preparation time was reduced, and the stability of the formed ph gradient was improved, compared with our previous methods. using the prepared m-ipg column, the baseline separation of proteins was achieved according to their pis. without carrier ampholytes added in the running buffer, the separated components could be detected with high sensitivity by uv at low wavelength. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | porous polymer monoliths ; capillary-zone-electrophoresis ; surface-chemistry ; chromatography ; electrochromatography ; digestion |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000297424500019 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142699]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Yu,Zhu, Guijie,Wang, Tingting,et al. Fast preparation of monolithic immobilized pH gradient column by photopolymerization and photografting techniques for isoelectric focusing separation of proteins[J]. electrophoresis,2011,32(20):2911-2914. |
| APA | Liang, Yu.,Zhu, Guijie.,Wang, Tingting.,Zhang, Xiaodan.,Liang, Zhen.,...&Zhang, Yukui.(2011).Fast preparation of monolithic immobilized pH gradient column by photopolymerization and photografting techniques for isoelectric focusing separation of proteins.electrophoresis,32(20),2911-2914. |
| MLA | Liang, Yu,et al."Fast preparation of monolithic immobilized pH gradient column by photopolymerization and photografting techniques for isoelectric focusing separation of proteins".electrophoresis 32.20(2011):2911-2914. |
入库方式： OAI收割
来源：大连化学物理研究所
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