Bioanalysis of Cepharanthine by LC-ESI-MS-MS and Its Application to Pharmacokinetic Studies
文献类型：期刊论文
| 作者 | Dong, Rui-Hua1; Fang, Zhong-Ze2,3; Gao, Hong-Zhi1; Hao, Guang-Tao1; Liu, Guang1; Shan, Ting-Ting1; Liu, Ze-Yuan1 |
| 刊名 | chromatographia
 |
| 出版日期 | 2011 |
| 卷号 | 73期号:1-2页码:75-81 |
| 关键词 | Column liquid chromatography-tandem mass spectrometry Pharmacokinetic study Cepharanthine |
| 英文摘要 | a sensitive liquid chromatography-electrospray ionization-tandem mass spectrometry (lc-esi-ms-ms) method was developed and validated for the quantification of cepharanthine (cep) in beagle dog plasma. the chromatographic separation was performed on an agilent-c18 column and the mobile phase was composed of methanol:water with 10 mm ammonium acetate (20:80, v/v). detection was operated in the positive ion mode and the tandem mass spectrometer was tuned in the multiple reactions monitoring mode (mrm) to monitor m/z transitions 607 -> 365 for cep and 285 -> 193 for the internal standard (is) diazepam. this method exhibited a linear range of 5-2,500 ng ml(-1). the precision (rsd%) and accuracy (rme%) of the assay were < 8.7 and 2.4%, respectively. the limit of quantification was 5 ng ml(-1) and no significant matrix effect was observed. the validated method has been successfully applied to pharmacokinetic study of cep in beagle dog. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | drug-metabolism ; discovery ; cells |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000286670500011 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142716]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Acad Mil Med Sci, Affiliated Hosp, Dept Clin Pharmacol, Beijing 100071, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, Rui-Hua,Fang, Zhong-Ze,Gao, Hong-Zhi,et al. Bioanalysis of Cepharanthine by LC-ESI-MS-MS and Its Application to Pharmacokinetic Studies[J]. chromatographia,2011,73(1-2):75-81. |
| APA | Dong, Rui-Hua.,Fang, Zhong-Ze.,Gao, Hong-Zhi.,Hao, Guang-Tao.,Liu, Guang.,...&Liu, Ze-Yuan.(2011).Bioanalysis of Cepharanthine by LC-ESI-MS-MS and Its Application to Pharmacokinetic Studies.chromatographia,73(1-2),75-81. |
| MLA | Dong, Rui-Hua,et al."Bioanalysis of Cepharanthine by LC-ESI-MS-MS and Its Application to Pharmacokinetic Studies".chromatographia 73.1-2(2011):75-81. |
入库方式： OAI收割
来源：大连化学物理研究所
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