Ultra-high capacity liquid chromatography chip/quadrupole time-of-flight mass spectrometry for pharmaceutical analysis
文献类型：期刊论文
| 作者 | Zhao, Chunxia1; Wu, Zeming1; Xue, Gang2; Wang, Jian2; Zhao, Yining2; Xu, Zhixiu3; Lin, Dayin3; Herbert, Godel3; Chang, Yuwei1; Cai, Kaiyang1 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2011-06-10 |
| 卷号 | 1218期号:23页码:3669-3674 |
| 关键词 | Ultra-high capacity chip Pharmaceutical analysis Liquid chromatography Mass spectrometry HPLC-Chip |
| 英文摘要 | nanoflow liquid chromatography/mass spectrometry (nano-lc/ms) has attracted increasing interest ill virtue of high sensitivity, low sample consumption, and minimal matrix effect. in this work a hplc-chip/quadrupole time-of-flight (q-tof) ms device with a new ultra-high capacity small molecule chip (uhc-chip) which features a 500 nl enrichment column and a 150 mm x 75 mu m analytical column, was evaluated with a drug mixture covering a wide range of polarities. excellent chromatographic precision with 0.1-0.5% rsd for retention time and 1.7-9.0% rsd for peak area, low limit of detection, good chip-to-chip reproducibility and linearity were obtained by using this uhc-chip. compared with the standard hplc-chip with 40 nl trapping column, the uhc-chip showed higher enrichment capability and hence gave a higher response in signal detection. additionally, 4-30 times increase in sensitivity was obtained compared with conventional lc/ms, which indicated that uhc-chip/ms was a valuable tool for the quantitative analysis of low level impurities and degradation products in pharmaceuticals. moreover, satisfactory results obtained from trace drug analysis of serum samples further proved its practicality and potential for use in drug testing and development. (c) 2011 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | integrated microfluidic device ; drug-delivery ; hplc-chip ; identification ; proteomics ; particles ; lc/ms |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000291521200013 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142721]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Pfizer Inc, Pfizer Global R&D, Groton, CT 06340 USA 3.Agilent Technol, Waldbronn, Germany |
| 推荐引用方式 GB/T 7714 | Zhao, Chunxia,Wu, Zeming,Xue, Gang,et al. Ultra-high capacity liquid chromatography chip/quadrupole time-of-flight mass spectrometry for pharmaceutical analysis[J]. journal of chromatography a,2011,1218(23):3669-3674. |
| APA | Zhao, Chunxia.,Wu, Zeming.,Xue, Gang.,Wang, Jian.,Zhao, Yining.,...&Xu, Guowang.(2011).Ultra-high capacity liquid chromatography chip/quadrupole time-of-flight mass spectrometry for pharmaceutical analysis.journal of chromatography a,1218(23),3669-3674. |
| MLA | Zhao, Chunxia,et al."Ultra-high capacity liquid chromatography chip/quadrupole time-of-flight mass spectrometry for pharmaceutical analysis".journal of chromatography a 1218.23(2011):3669-3674. |
入库方式： OAI收割
来源：大连化学物理研究所
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