Regioselective Galactosylation of Floxuridine Catalyzed by beta-Galactosidase from Bovine Liver in Co-solvent Systems
文献类型：期刊论文
| 作者 | Ye Min2; Liu Qiuping2; Li Ning1,3; Zong Minhua1 |
| 刊名 | chinese journal of catalysis
 |
| 出版日期 | 2011-06-01 |
| 卷号 | 32期号:6页码:1063-1068 |
| 关键词 | beta-galactosidase glycosylation regioselectivity floxuridine organic solvent |
| 英文摘要 | the influence of organic solvents and their contents on the regioselective galactosylation of floxuridine catalyzed by if-galactosidase from bovine liver was investigated in co-solvent systems. it was revealed that the enzyme was unstable and inactivated substantially in organic solvent (10%, v/v)-containing systems, and the detrimental impacts of organic solvents on the enzyme could be relieved remarkably by decreasing their contents. nevertheless, the addition of organic solvents exerted no influence on the regioselectivity of the enzymatic glycosylation, which retained >99%. the catalytic performance of beta-galactosidase from bovine liver correlated well with the dielectric constants of organic solvents in co-solvent systems, among which the enzyme displayed the highest activity in dimethyl sulfoxide (dmso)-containing system. the yield of desired product reached 71% when the dmso content was less than 5% (v/v) in the medium. |
| WOS标题词 | science & technology ; physical sciences ; technology |
| 类目[WOS] | chemistry, applied ; chemistry, physical ; engineering, chemical |
| 研究领域[WOS] | chemistry ; engineering |
| 关键词[WOS] | disaccharide nucleosides ; n-acetyllactosamine ; pyrococcus-furiosus ; organic-solvents ; glucosidase ; media ; 5-fluorouridine ; stability |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000292429000026 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142735]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.S China Univ Technol, Coll Light Ind & Food Sci, State Key Lab Pulp & Paper Engn, Guangzhou 510640, Guangdong, Peoples R China 2.S China Univ Technol, Sch Biosci & Bioengn, Guangzhou 510006, Guangdong, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Liaoning, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye Min,Liu Qiuping,Li Ning,et al. Regioselective Galactosylation of Floxuridine Catalyzed by beta-Galactosidase from Bovine Liver in Co-solvent Systems[J]. chinese journal of catalysis,2011,32(6):1063-1068. |
| APA | Ye Min,Liu Qiuping,Li Ning,&Zong Minhua.(2011).Regioselective Galactosylation of Floxuridine Catalyzed by beta-Galactosidase from Bovine Liver in Co-solvent Systems.chinese journal of catalysis,32(6),1063-1068. |
| MLA | Ye Min,et al."Regioselective Galactosylation of Floxuridine Catalyzed by beta-Galactosidase from Bovine Liver in Co-solvent Systems".chinese journal of catalysis 32.6(2011):1063-1068. |
入库方式： OAI收割
来源：大连化学物理研究所
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