CENP-E Kinesin Interacts with SKAP Protein to Orchestrate Accurate Chromosome Segregation in Mitosis
文献类型：期刊论文
| 作者 | Huang, Yuejia1,2; Wang, Wenwen1; Yao, Phil2; Wang, Xiwei1,2; Liu, Xing1,2; Zhuang, Xiaoxuan1; Yan, Feng1,2; Zhou, Jinhua1; Du, Jian1; Ward, Tarsha2 |
| 刊名 | journal of biological chemistry
 |
| 出版日期 | 2012-01-06 |
| 卷号 | 287期号:2页码:1500-1509 |
| 英文摘要 | mitotic chromosome segregation is orchestrated by the dynamic interaction of spindle microtubules with the kinetochore. although previous studies show that the mitotic kinesin cenp-e forms a link between attachment of the spindle microtubule to the kinetochore and the mitotic checkpoint signaling cascade, the molecular mechanism underlying dynamic kinetochore-microtubule interactions in mammalian cells remains elusive. here, we identify a novel interaction between cenp-e and skap that functions synergistically in governing dynamic kinetochore-microtubule interactions. skap binds to the c-terminal tail of cenp-e in vitro and is essential for an accurate kinetochore-microtubule attachment in vivo. immunoelectron microscopic analysis indicates that skap is a constituent of the kinetochore corona fibers of mammalian centromeres. depletion of skap or cenp-e by rna interference results in a dramatic reduction of inter-kinetochore tension, which causes chromosome mis-segregation with a prolonged delay in achieving metaphase alignment. importantly, skap binds to microtubules in vitro, and this interaction is synergized by cenp-e. based on these findings, we propose that skap cooperates with cenp-e to orchestrate dynamic kinetochore-microtubule interaction for faithful chromosome segregation. |
| 类目[WOS] | biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | microtubule plus ends ; mitotic checkpoint ; spindle microtubules ; kinetochore protein ; outer plate ; attachment ; localization ; motor ; aneuploidy ; complex |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000299170300063 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142833]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Univ Sci & Technol China, Anhui Key Lab Cellular Dynam & Chem Biol, Sch Life Sci, Hefei 230027, Peoples R China 2.Morehouse Sch Med, Dept Physiol, Atlanta, GA 30310 USA 3.Dalian Inst Chem Phys, Dalian 116023, Peoples R China 4.Beijing Univ Chinese Med, Sch Grad Studies, Beijing 100029, Peoples R China 5.Guangzhou Inst Hlth Sci, Guangzhou 510515, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Yuejia,Wang, Wenwen,Yao, Phil,et al. CENP-E Kinesin Interacts with SKAP Protein to Orchestrate Accurate Chromosome Segregation in Mitosis[J]. journal of biological chemistry,2012,287(2):1500-1509. |
| APA | Huang, Yuejia.,Wang, Wenwen.,Yao, Phil.,Wang, Xiwei.,Liu, Xing.,...&Yao, Xuebiao.(2012).CENP-E Kinesin Interacts with SKAP Protein to Orchestrate Accurate Chromosome Segregation in Mitosis.journal of biological chemistry,287(2),1500-1509. |
| MLA | Huang, Yuejia,et al."CENP-E Kinesin Interacts with SKAP Protein to Orchestrate Accurate Chromosome Segregation in Mitosis".journal of biological chemistry 287.2(2012):1500-1509. |
入库方式： OAI收割
来源：大连化学物理研究所
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