Screening of Metabolite Regulating Lactic Acid Production of Exiguobacterium aurantiacum ATCC49676 by Metabolic Fingerprint Analysis
文献类型：期刊论文
| 作者 | Lian Rongwei1,2; Tian Jing1; Gao Peng2; Wang Xiyue2; Fei Xu1; Wang Yi1; Xu Guowang2 |
| 刊名 | acta chimica sinica
 |
| 出版日期 | 2012-12-28 |
| 卷号 | 70期号:24页码:2513-2517 |
| 关键词 | metabolic fingerprint analysis lactic acid GC-MS L-glutamic acid LDH |
| 英文摘要 | a strain of exiguobacterium aurantiacum atcc49676 was found to be of great potential to produce lactic acid (la). in order to screen the metabolites that might regulate or affect the acid yield, a relative higher la production condition was defined through full factorial experiment design. fresh cultures harvested from the basic and the higher la production conditions were subjected to metabolic fingerprint analysis by gas chromatography-mass spectrometry. significance analysis indicated a distinct change of an intercellular metabolite-l-glutamic acid (glu). when atcc49676 was treated by glu, la yield declined with the increasing added glu concentrations. relative enzyme quantification confirmed that glu decreased the intracellular lactate dehydrogenase content. this study proved the merit of metabolic fingerprint analysis in exploring the phenotype specific intracellular metabolite and its potential roles in improving industrial fermentation efficiencies. |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | microbial metabolomics ; functional genomics ; mass-spectrometry ; extremophiles ; optimization |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000314308100008 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142878]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Polytech Univ, Sch Bioengn, Dalian 116034, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lian Rongwei,Tian Jing,Gao Peng,et al. Screening of Metabolite Regulating Lactic Acid Production of Exiguobacterium aurantiacum ATCC49676 by Metabolic Fingerprint Analysis[J]. acta chimica sinica,2012,70(24):2513-2517. |
| APA | Lian Rongwei.,Tian Jing.,Gao Peng.,Wang Xiyue.,Fei Xu.,...&Xu Guowang.(2012).Screening of Metabolite Regulating Lactic Acid Production of Exiguobacterium aurantiacum ATCC49676 by Metabolic Fingerprint Analysis.acta chimica sinica,70(24),2513-2517. |
| MLA | Lian Rongwei,et al."Screening of Metabolite Regulating Lactic Acid Production of Exiguobacterium aurantiacum ATCC49676 by Metabolic Fingerprint Analysis".acta chimica sinica 70.24(2012):2513-2517. |
入库方式： OAI收割
来源：大连化学物理研究所
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