Comprehensive and Highly Sensitive Urinary Steroid Hormone Profiling Method Based on Stable Isotope-Labeling Liquid Chromatography Mass Spectrometry
文献类型：期刊论文
| 作者 | Dai, Weidong; Huang, Qiang; Yin, Peiyuan; Li, Jia; Zhou, Jia; Kong, Hongwei; Zhao, Chunxia; Lu, Xin; Xu, Guowang |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2012-12-04 |
| 卷号 | 84期号:23页码:10245-10251 |
| 英文摘要 | steroid hormones are crucial substances that mediate a wide range of vital physiological functions of the body. determination of the levels of steroid hormones plays an important role in understanding the mechanism of the steroid hormone-related diseases. in this study, we present a novel targeted metabolic profiling method based on the introduction of an easily protonated stable isotope tag to a hydroxyl-containing steroid hormone with a synthesized derivatization reagent, deuterium 4-(dimethylamino)-benzoic acid (d(4)-dmba), and liquid chromatography mass spectrometry (lc-ms). different from other reported derivatization reagents that have been used to enhance the sensitivities for estrogens or androgens, our method is comprehensive with the capability of covering hydroxyl-containing androgens, estrogens, corticoids, and progestogens. furthermore, the nonderivatized steroid hormones (e.g., 17 alpha-hydroxyprogesterone, progesterone, and androstenedione) were not destroyed during the derivatization process, and their levels could still be obtained in one lc-ms run. we were able to detect 2.4 steroid hormones at subng/ml levels (the lower limit of detection could reach 5 pg/ml for estrone and 16 alpha-hydroxy estrone, which is equivalent to 0.1 pg on column) with maximum sensitivity enhancement factors of more than 10(3)- to 10(4)-fold after derivatization. the method was successfully applied to the measurement of free (unconjugated) steroid hormones in urine samples of males, females, and pregnant women. because the significant role the steroid hormone pathway plays in humans, a comprehensive, sensitive, specific, and accurate method for profiling the steroid hormone metabolome shall offer new insights into hormone-related diseases. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | prostate-cancer ; breast-cancer ; relative quantification ; quantitative proteomics ; containing metabolites ; rheumatoid-arthritis ; endogenous estrogens ; human serum ; derivatization ; risk |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000311815300016 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142980]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dai, Weidong,Huang, Qiang,Yin, Peiyuan,et al. Comprehensive and Highly Sensitive Urinary Steroid Hormone Profiling Method Based on Stable Isotope-Labeling Liquid Chromatography Mass Spectrometry[J]. analytical chemistry,2012,84(23):10245-10251. |
| APA | Dai, Weidong.,Huang, Qiang.,Yin, Peiyuan.,Li, Jia.,Zhou, Jia.,...&Xu, Guowang.(2012).Comprehensive and Highly Sensitive Urinary Steroid Hormone Profiling Method Based on Stable Isotope-Labeling Liquid Chromatography Mass Spectrometry.analytical chemistry,84(23),10245-10251. |
| MLA | Dai, Weidong,et al."Comprehensive and Highly Sensitive Urinary Steroid Hormone Profiling Method Based on Stable Isotope-Labeling Liquid Chromatography Mass Spectrometry".analytical chemistry 84.23(2012):10245-10251. |
入库方式： OAI收割
来源：大连化学物理研究所
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