SDS-PAGE-free protocol for comprehensive identification of cytochrome P450 enzymes and uridine diphosphoglucuronosyl transferases in human liver microsomes
文献类型：期刊论文
| 作者 | Sun, Liangliang1; Zhang, Yanyan2; Tao, Dingyin1; Zhu, Guijie1; Zhao, Qun1,3; Wu, Qi1,3; Liang, Zhen1; Yang, Ling2; Zhang, Lihua1; Zhang, Yukui1 |
| 刊名 | proteomics
 |
| 出版日期 | 2012-12-01 |
| 卷号 | 12期号:23-24页码:3464-3469 |
| 关键词 | 1-Butyl-3-methyl imidazolium tetrafluoroborate 2D-LC-ESI-MS/MS Cytochrome P450 enzyme Protein spectral count Uridine diphosphoglucuronosyl transferase |
| 英文摘要 | comprehensive identification of cytochrome p450 enzymes (cyps) and uridine diphosphoglucuronosyl transferases (ugts) in human liver microsomes (hlms) was performed with an sds-page-free protocol. hlms were solubilized with 5% v/v ionic liquid, 1-butyl-3-methyl imidazolium tetrafluoroborate, followed by tryptic digestion, and 2d-scx-rplc-esi-ms/ms (ltq xl) analysis in triplicate. in total, 27 cyps and 12 ugts were confidently identified with average sequence coverage as 30.99 and 25.07%, average peptide number as 14 and 13, and average unique peptide number as 7 and 4, respectively. the highly similar isoforms of cyp3a, cyp2c, and cyp4f subfamilies could be unambiguously differentiated from each other, despite the fact that the sequence similarity of cyp2c9 and cyp2c19 is 91%. in addition, protein spectral count was used to approximately evaluate the relative abundance of identified cyps and ugts, and the results agreed with previous immunochemistry reports. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemical research methods ; biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | tandem mass-spectrometry ; proteomic approach ; isoforms ; p450 ; quantification ; therapeutics ; expression ; tissue |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000312650100003 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/143063]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab High Efficient Separat & High Sensit Characte, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Liangliang,Zhang, Yanyan,Tao, Dingyin,et al. SDS-PAGE-free protocol for comprehensive identification of cytochrome P450 enzymes and uridine diphosphoglucuronosyl transferases in human liver microsomes[J]. proteomics,2012,12(23-24):3464-3469. |
| APA | Sun, Liangliang.,Zhang, Yanyan.,Tao, Dingyin.,Zhu, Guijie.,Zhao, Qun.,...&Zhang, Yukui.(2012).SDS-PAGE-free protocol for comprehensive identification of cytochrome P450 enzymes and uridine diphosphoglucuronosyl transferases in human liver microsomes.proteomics,12(23-24),3464-3469. |
| MLA | Sun, Liangliang,et al."SDS-PAGE-free protocol for comprehensive identification of cytochrome P450 enzymes and uridine diphosphoglucuronosyl transferases in human liver microsomes".proteomics 12.23-24(2012):3464-3469. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。