Characterization of the mitochondrial NAD(+)-dependent isocitrate dehydrogenase of the oleaginous yeast Rhodosporidium toruloides
文献类型：期刊论文
| 作者 | Yang, Fan1,2; Zhang, Sufang1; Zhou, Yongjin J.1; Zhu, Zhiwei1; Lin, Xinping1; Zhao, Zongbao K.1,3 |
| 刊名 | applied microbiology and biotechnology
 |
| 出版日期 | 2012-05-01 |
| 卷号 | 94期号:4页码:1095-1105 |
| 关键词 | Rhodosporidium toruloides Microbial lipid NAD(+)-specific isocitrate dehydrogenase Nitrogen limitation Oleaginous yeast |
| 英文摘要 | early biochemical studies have demonstrated that lipid accumulation by oleaginous yeasts is linked to the activity of the nad(+)-dependent isocitrate dehydrogenase (idh). however, molecular study of idh of oleaginous microorganisms remains limited. here, we present the cloning of a mitochondrial nad(+)-specific idh from rhodosporidium toruloides (rtidh), an excellent microbial lipid producer that uses carbohydrates as the carbon source. the evolutionary relationship analyses among rtidhs and other yeast idhs revealed that rtidh had a closer relationship with the idhs of ustilago maydis and schizophyllum commune. we expressed the rtidh gene in the yeast saccharomyces cerevisiae idh delta mutant. under the nitrogen-limited condition, the intracellular lipid content and extracellular citrate concentration of the culture of the s. cerevisiae idh delta carrying the rtidh gene increased as the carbon/nitrogen molar ratio of the media increased, while the wild-type s. cerevisiae strain showed no correlation. our data provided valuable information for elucidating the molecular mechanism of microbial oleaginicity and for engineering microorganisms to produce metabolites of fatty acid pathway. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | lipid-accumulation ; saccharomyces-cerevisiae ; binding-sites ; yarrowia-lipolytica ; gene ; acid ; disruption ; metabolism ; fragments ; products |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000303544400024 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/143085]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Div Biotechnol, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Dalian Polytech Univ, Sch Biol Engn, Dalian 116034, Peoples R China 3.Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Fan,Zhang, Sufang,Zhou, Yongjin J.,et al. Characterization of the mitochondrial NAD(+)-dependent isocitrate dehydrogenase of the oleaginous yeast Rhodosporidium toruloides[J]. applied microbiology and biotechnology,2012,94(4):1095-1105. |
| APA | Yang, Fan,Zhang, Sufang,Zhou, Yongjin J.,Zhu, Zhiwei,Lin, Xinping,&Zhao, Zongbao K..(2012).Characterization of the mitochondrial NAD(+)-dependent isocitrate dehydrogenase of the oleaginous yeast Rhodosporidium toruloides.applied microbiology and biotechnology,94(4),1095-1105. |
| MLA | Yang, Fan,et al."Characterization of the mitochondrial NAD(+)-dependent isocitrate dehydrogenase of the oleaginous yeast Rhodosporidium toruloides".applied microbiology and biotechnology 94.4(2012):1095-1105. |
入库方式： OAI收割
来源：大连化学物理研究所
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