Comprehensive characterization of Stevia Rebaudiana using two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography
文献类型：期刊论文
| 作者 | Fu, Qing1,2; Guo, Zhimou2; Zhang, Xiuli2; Liu, Yanfang2; Liang, Xinmiao1,2 |
| 刊名 | journal of separation science
 |
| 出版日期 | 2012-07-01 |
| 卷号 | 35期号:14页码:1821-1827 |
| 关键词 | Characterization HILIC RP Stevia rebaudiana Two-dimensional liquid chromatography (2D-LC) |
| 英文摘要 | two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography (2d-rplc/hilic) system was successfully applied for comprehensive characterization of steviol glycosides from stevia rebaudiana. the experiments were performed in offline mode using an xcharge c18 column in first dimension and an xamide column in second dimension. in first dimension, preliminary separation of stevia aqueous extract was accomplished and 30 fractions were collected. then fractions 120 were selected for further purification and 13 compounds with high purity were obtained in second dimension. comprehensive characterization of these compounds was completed by determination of their retention time, accurate molecular weight, diagnostic fragmentation ions, and nuclear magnetic resonance spectroscopy. as a result, all nine known steviol glycosides, as well as other four steviol glycosides were fully purified. the result demonstrated that this procedure is an effective approach for the preparative separation and comprehensive characterization of steviol glycosides in stevia. this 2d-rplc/hilic method will be a promising tool for the purification of low-abundance compounds from natural products. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | mass-spectrometry ; fluid extraction ; plant-origin ; stevioside ; glycosides ; separation ; peptides ; leaves ; sweeteners ; bertoni |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000306493700015 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/143118]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Nat Med Liaoning Prov, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, Qing,Guo, Zhimou,Zhang, Xiuli,et al. Comprehensive characterization of Stevia Rebaudiana using two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography[J]. journal of separation science,2012,35(14):1821-1827. |
| APA | Fu, Qing,Guo, Zhimou,Zhang, Xiuli,Liu, Yanfang,&Liang, Xinmiao.(2012).Comprehensive characterization of Stevia Rebaudiana using two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography.journal of separation science,35(14),1821-1827. |
| MLA | Fu, Qing,et al."Comprehensive characterization of Stevia Rebaudiana using two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography".journal of separation science 35.14(2012):1821-1827. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。