Oxidative Decarboxylation of L-Malate by Using a Synthetic Bioredox System
文献类型：期刊论文
| 作者 | Ji Debin2; Wang Lei2; Zhou Yongjin2; Yang Wei; Wang Qian1; Zhao Zongbao1 |
| 刊名 | chinese journal of catalysis
 |
| 出版日期 | 2012-03-01 |
| 卷号 | 33期号:3页码:530-535 |
| 关键词 | biocatalysis crude cell lysate malic enzyme pyruvate synthetic bioredox system |
| 英文摘要 | a synthetic bioredox system for the oxidative decarboxylation of l-malate to pyruvate was presented, with which the enzyme purification steps can be omitted. the bioredox system consisted of me-t (mutant malic enzyme me l310r/q401c) and non-natural coenzyme nicotinamide flucytosine dinucleotide (nfcd+) catalyzes the oxidation of l-malate to pyruvate and concurrently generates the reduced coenzyme, nfcdh. escherichia coli bl21(de3) pet24b-me-t cell lysates catalyze the oxidation of l-malate and produce pyruvate only, indicating that the cell lysates have the same substrate selectivity as the purified enzyme. nadh oxidase (nox) from enterococcus faecalis can oxidize nfcdh to regenerate nfcd+. compared with the reaction system containing nad(+), me lysates, and nox lysates, the system containing nfcd+, me-t lysates, and nox lysates converts l-malate to pyruvate in 9% higher yield and with significantly less lactate formation. these results suggest that the synthetic bioredox system composed of me-t and nfcd+ is easy to use and has higher product selectivity than the corresponding natural system dependent on nad(+). the synthetic bioredox system has the potential to substitute the purified enzyme. the results provide new opportunities for design and application of redox biocatalysts. |
| WOS标题词 | science & technology ; physical sciences ; technology |
| 类目[WOS] | chemistry, applied ; chemistry, physical ; engineering, chemical |
| 研究领域[WOS] | chemistry ; engineering |
| 关键词[WOS] | nadh oxidase ; cofactor regeneration ; stereochemical control ; enzymatic reduction ; pyruvate production ; enzymes ; purification ; expression ; yeast |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000302114300021 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/143134]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian Natl Lab Clean Energy, Dalian 116023, Liaoning, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ji Debin,Wang Lei,Zhou Yongjin,et al. Oxidative Decarboxylation of L-Malate by Using a Synthetic Bioredox System[J]. chinese journal of catalysis,2012,33(3):530-535. |
| APA | Ji Debin,Wang Lei,Zhou Yongjin,Yang Wei,Wang Qian,&Zhao Zongbao.(2012).Oxidative Decarboxylation of L-Malate by Using a Synthetic Bioredox System.chinese journal of catalysis,33(3),530-535. |
| MLA | Ji Debin,et al."Oxidative Decarboxylation of L-Malate by Using a Synthetic Bioredox System".chinese journal of catalysis 33.3(2012):530-535. |
入库方式： OAI收割
来源：大连化学物理研究所
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