Liquid-liquid/solid three-phase high-speed counter-current chromatography, a new technique for separation of polyphenols from Geranium wilfordii Maxim
文献类型：期刊论文
| 作者 | Liu, Dan1,2; Ma, Yan3; Gu, Ming1; Janson, Jan-Christer4; Wang, Changhai5; Xiao, Hongbin2 |
| 刊名 | journal of separation science
 |
| 出版日期 | 2012-08-01 |
| 卷号 | 35期号:16页码:2146-2151 |
| 关键词 | Corilagin Geraniin High-speed counter-current chromatography Liquid-liquid solid three-phase system Polyphenols |
| 英文摘要 | high-speed counter-current chromatography using a new liquidliquid/solid three-phase system was used for the separation of the polyphenols corilagin and geraniin from a crude extract of geranium wilfordii maxim in one step. the optimized three-phase system was composed of n-hexane/ethyl acetate/methanol/acetic acid/water and to which was added 10-mu m average diameter microspheres of cross-linked 12% agarose at the ratio of 0.2:10:2:1:5 and 0.1 g/ml, respectively. the purities of geraniin and corilagin were 82 and 90%, which were determined by hplc at 280 nm. a 14.5 and 7 mg of geraniin and corilagin were purified from 160 mg crude extract with the yields of 70 and 78%, respectively. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | linked 12-percent agarose ; one-step purification ; bond adsorption chromatography ; salvianolic acid-b ; 3,4-dihydroxyphenyllactic acid ; phyllanthus-urinaria ; hydrolyzable tannins ; gel media ; corilagin ; protocatechualdehyde |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000307551300020 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/143147]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China 3.Liaoning Prov Dalian Ocean Fishery Grp Corp, Dalian, Peoples R China 4.Uppsala Univ, Dept Phys & Analyt Chem, Uppsala, Sweden 5.Nanjing Agr Univ, Nanjing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Dan,Ma, Yan,Gu, Ming,et al. Liquid-liquid/solid three-phase high-speed counter-current chromatography, a new technique for separation of polyphenols from Geranium wilfordii Maxim[J]. journal of separation science,2012,35(16):2146-2151. |
| APA | Liu, Dan,Ma, Yan,Gu, Ming,Janson, Jan-Christer,Wang, Changhai,&Xiao, Hongbin.(2012).Liquid-liquid/solid three-phase high-speed counter-current chromatography, a new technique for separation of polyphenols from Geranium wilfordii Maxim.journal of separation science,35(16),2146-2151. |
| MLA | Liu, Dan,et al."Liquid-liquid/solid three-phase high-speed counter-current chromatography, a new technique for separation of polyphenols from Geranium wilfordii Maxim".journal of separation science 35.16(2012):2146-2151. |
入库方式： OAI收割
来源：大连化学物理研究所
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