Evaluation and comparison of n-alkyl chain and polar ligand bonded stationary phases for protein separation in reversed-phase liquid chromatography
文献类型：期刊论文
| 作者 | Ding, Ling; Guo, Zhimou; Xiao, Yuansheng; Xue, Xingya; Zhang, Xiuli; Liang, Xinmiao |
| 刊名 | journal of separation science
 |
| 出版日期 | 2014-09-01 |
| 卷号 | 37期号:18页码:2467-2473 |
| 关键词 | Alkyl chains Polar ligands Protein separation Retention equations Reversed-phase liquid chromatography |
| 英文摘要 | protein retention is very sensitive to the change of solvent composition in reversed-phase liquid chromatography for so called "on-off" mechanism, leading to difficulty in mobile phase optimization. in this study, a novel 3-chloropropyl trichlorosilane ligand bonded column was prepared for protein separation. the differences in retention characteristics between t he 3-chloropropyl trichlorosilane ligand bonded column and n-alkyl chainmodified (c-2, c-4, c-8) stationary phases were elucidated by the retention equation ln k = a + cc(b). retention parameters (a and c) of nine standard proteins with different molecular weights were calculated by using homemade software. results showed that retention times of nine proteins were similar on four columns, but the 3-chloropropyl trichlorosilane ligand bonded column obtained the lowest retention parameter values of larger proteins. it meant that their retention behavior affected by acetonitrile concentration would be different due to lower vertical bar c vertical bar values. more specifically, protein elution windows were broader, and retentions were less sensitive to the change of acetonitrile concentration on the 3-chloropropyl trichlorosilane ligand bonded column than that on other columns. meanwhile, the 3-chloropropyl trichlorosilane ligand bonded column displayed distinctive selectivity for some proteins. our results indicated that stationary phase with polar ligand provided potential solutions to the "on-off" problem and optimization in protein separation. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | gradient elution ; horizontal polymerization ; exchange chromatography ; retention equation ; intact proteins ; performance ; silica ; peptides ; packings ; hplc |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000342909000002 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/143196]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Ling,Guo, Zhimou,Xiao, Yuansheng,et al. Evaluation and comparison of n-alkyl chain and polar ligand bonded stationary phases for protein separation in reversed-phase liquid chromatography[J]. journal of separation science,2014,37(18):2467-2473. |
| APA | Ding, Ling,Guo, Zhimou,Xiao, Yuansheng,Xue, Xingya,Zhang, Xiuli,&Liang, Xinmiao.(2014).Evaluation and comparison of n-alkyl chain and polar ligand bonded stationary phases for protein separation in reversed-phase liquid chromatography.journal of separation science,37(18),2467-2473. |
| MLA | Ding, Ling,et al."Evaluation and comparison of n-alkyl chain and polar ligand bonded stationary phases for protein separation in reversed-phase liquid chromatography".journal of separation science 37.18(2014):2467-2473. |
入库方式： OAI收割
来源：大连化学物理研究所
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