Establishment of a new OSCC cell line derived from OLK and identification of malignant transformation-related proteins by differential proteomics approach
文献类型：期刊论文
| 作者 | Dong, Yan1,2,3; Zhao, Qun4; Ma, Xiaoyan1,2,3; Ma, Guowu1; Liu, Caiyun1,2,3; Chen, Zhuwen1; Yu, Liyuan1,2,3; Liu, Xuefeng5; Zhang, Yanguang1,2,3; Shao, Shujuan5 |
| 刊名 | scientific reports
 |
| 出版日期 | 2015-08-03 |
| 卷号 | 5期号:1页码:1 |
| 通讯作者 | yandong ; 赵群 |
| 英文摘要 | oral squamous cell carcinoma (oscc) is usually preceded by the oral premalignant lesions, mainly oral leukoplakia (olk) after repeated insults of carcinogens, tobacco. b(a)p and dmba are key carcinogens in tobacco smoke. in the present study, for the first time we established the cancerous cell line oscc-bd induced by b(a)p/dmba mixture and transformed from dysplastic oral leukoplakia cell line dok. cell morphology, proliferation ability, migration ability, colony formation, and tumorigenicity were studied and confirmed the malignant characteristics of oscc-bd cells. we further identified the differential proteins between dok and oscc-bd cells by stable isotope dimethyl labeling based quantitative proteomic method, which showed 18 proteins up-regulated and 16 proteins down-regulated with rsd < 8%. differential proteins are mainly related to cell cycle, cell proliferation, dna replication, rna splicing and apoptosis. abberant binding function, catalysis activity and transportor activity of differential proteins might contribute to the malignant transformation of olk. of the 34 identified differential proteins with rsd < 8%, 13 novel cancer-related proteins were reported in the present study. this study might provide a new insight into the mechanism of olk malignant transformation and the potent biomarkers for early diagnosis, meanwhile further facilitate the application of the quantification proteomics to carcinogenesis research. |
| 学科主题 | 物理化学 |
| WOS标题词 | science & technology |
| 类目[WOS] | multidisciplinary sciences |
| 研究领域[WOS] | science & technology - other topics |
| 关键词[WOS] | breast-cancer ; mcm4 expression ; carcinoma ; overexpression ; quantification ; carcinogenesis ; increase ; marker |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000358856000001 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/143688]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Med Univ, Coll Stomatol, Dalian 116044, Peoples R China 2.Chinese Acad Med Sci, State Key Lab Mol Oncol, Beijing 100021, Peoples R China 3.Peking Union Med Coll, Beijing 100021, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 5.Dalian Med Univ, Affiliated Hosp 2, Ctr Canc, Inst Canc Stem Cell, Dalian 116044, Peoples R China 6.Dalian Univ Technol, Dept Engn Mech, Dalian 116023, Peoples R China 7.Chinese Acad Med Sci, Canc Hosp, Dept Pathol, Beijing 100021, Peoples R China 8.Chinese Acad Med Sci, Inst Canc, Beijing 100021, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, Yan,Zhao, Qun,Ma, Xiaoyan,et al. Establishment of a new OSCC cell line derived from OLK and identification of malignant transformation-related proteins by differential proteomics approach[J]. scientific reports,2015,5(1):1. |
| APA | Dong, Yan.,Zhao, Qun.,Ma, Xiaoyan.,Ma, Guowu.,Liu, Caiyun.,...&Zhang, Lihua.(2015).Establishment of a new OSCC cell line derived from OLK and identification of malignant transformation-related proteins by differential proteomics approach.scientific reports,5(1),1. |
| MLA | Dong, Yan,et al."Establishment of a new OSCC cell line derived from OLK and identification of malignant transformation-related proteins by differential proteomics approach".scientific reports 5.1(2015):1. |
入库方式： OAI收割
来源：大连化学物理研究所
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