Epitope imprinted polymer nanoparticles containing fluorescent quantum dots for specific recognition of human serum albumin
文献类型：期刊论文
| 作者 | Wang, Yi-Zhi1,2; Li, Dong-Yan1,2; He, Xi-Wen1,2; Li, Wen-You1,2; Zhang, Yu-Kui1,2,3 |
| 刊名 | microchimica acta
 |
| 出版日期 | 2015-06-01 |
| 卷号 | 182期号:7-8页码:1465-1472 |
| 关键词 | Epitope imprinting Human serum albumin Quantum dots Turn-on fluorescence Fluorometry |
| 通讯作者 | 李文友 ; 张玉奎 |
| 英文摘要 | epitope imprinted polymer nanoparticles (ei-nps) were prepared by one-pot polymerization of n-isopropylacrylamide in the presence of cdte quantum dots and an epitope (consisting of amino acids 598 to 609) of human serum albumin (hsa). the resulting ei-nps exhibit specific recognition ability and enable direct fluorescence quantification of hsa based on a fluorescence turn-on mode. the polymer was characterized by ft-ir, x-ray photoelectron spectroscopy, transmission electron microscopy and dynamic light scattering. the linear calibration graph was obtained in the range of 0.25-5 mu mol center dot ml(-1) with the detection limit of 44.3 nmol center dot ml(-1). the ei-nps were successfully applied to the direct fluorometric quantification of hsa in samples of human serum. overall, this approach provides a promising tool to design functional fluorescent materials with protein recognition capability and specific applications in proteomics. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | quartz-crystal microbalance ; doped zns qds ; target protein ; proteomic analysis ; surface ; acid ; nanocrystals ; quantification ; resolution ; biomarkers |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000354414300029 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/143695]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nankai Univ, State Key Lab Med Chem Biol, Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China 2.Nankai Univ, Res Ctr Analyt Sci, Coll Chem, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yi-Zhi,Li, Dong-Yan,He, Xi-Wen,et al. Epitope imprinted polymer nanoparticles containing fluorescent quantum dots for specific recognition of human serum albumin[J]. microchimica acta,2015,182(7-8):1465-1472. |
| APA | Wang, Yi-Zhi,Li, Dong-Yan,He, Xi-Wen,Li, Wen-You,&Zhang, Yu-Kui.(2015).Epitope imprinted polymer nanoparticles containing fluorescent quantum dots for specific recognition of human serum albumin.microchimica acta,182(7-8),1465-1472. |
| MLA | Wang, Yi-Zhi,et al."Epitope imprinted polymer nanoparticles containing fluorescent quantum dots for specific recognition of human serum albumin".microchimica acta 182.7-8(2015):1465-1472. |
入库方式： OAI收割
来源：大连化学物理研究所
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