Particulate capillary precolumns with double-end polymer monolithic frits for on-line peptide trapping and preconcentration
文献类型：期刊论文
| 作者 | Xia, Si-Min1,2; Yuan, Hui-Ming2; Liang, Zheng2; Zhang, Li-Hua2; Zhang, Yu-Kui2 |
| 刊名 | chinese chemical letters
 |
| 出版日期 | 2015-09-01 |
| 卷号 | 26期号:9页码:1068-1072 |
| 关键词 | Capillary precolumn Double-end frits Peptide trapping 2D nano-LC |
| 通讯作者 | 张丽华 |
| 英文摘要 | in this work, a novel kind of particulate capillary precolumns with double-end polymer monolithic frits has been developed. firstly, the polymer monolithic frit at one end was prepared via photo-initiated polymerization of a mixture of lauryl methacrylate and ethyleneglycol dimethacrylate with 1-propanol and 1,4-butanediol as porogens and 2,2-dimethoxy-2-phenylacetophenone as a photo-initiator in uv transparent coating capillary (100 mu m i.d.). subsequently, c18 particles (5 mu m, 100 angstrom) were packed into the capillary, and sealed with the polymer monolithic frit at another end. to prevent the reaction of monomers and c18 particles, the packed c18 particles were masked during uv exposure. the loading capacity of such a precolumn was determined to be about 9 mu g by frontal analysis with a synthetic peptide apgdriyvhpf as a model sample. furthermore, two parallel precolumns were incorporated into a two-dimensional nano-liquid chromatography (2d nano-lc) system with dual capillary trap columns for peptide trapping and concentration. compared to 2d nano-lc system with a single trap column, such two dimensional separations could be operated simultaneously to improve the analysis throughput. all these results demonstrated that such capillary precolumns with double frits would be promising for high-throughput proteome analysis. (c) 2015 chinese chemical society and institute of materia medica, chinese academy of medical sciences. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | solid-phase extraction ; mass-spectrometry ; liquid-chromatography ; proteome analysis ; identification ; columns ; system ; electrochromatography |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000361407700004 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/143706]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xia, Si-Min,Yuan, Hui-Ming,Liang, Zheng,et al. Particulate capillary precolumns with double-end polymer monolithic frits for on-line peptide trapping and preconcentration[J]. chinese chemical letters,2015,26(9):1068-1072. |
| APA | Xia, Si-Min,Yuan, Hui-Ming,Liang, Zheng,Zhang, Li-Hua,&Zhang, Yu-Kui.(2015).Particulate capillary precolumns with double-end polymer monolithic frits for on-line peptide trapping and preconcentration.chinese chemical letters,26(9),1068-1072. |
| MLA | Xia, Si-Min,et al."Particulate capillary precolumns with double-end polymer monolithic frits for on-line peptide trapping and preconcentration".chinese chemical letters 26.9(2015):1068-1072. |
入库方式： OAI收割
来源：大连化学物理研究所
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