Integrated SDS removal and protein digestion by hollow fiber membrane based device for SDS-assisted proteome analysis
文献类型：期刊论文
| 作者 | Xia, Simin1,2; Yuan, Huiming1; Chen, Yuanbo1,2; Liang, Zheng1; Zhang, Lihua1; Zhang, Yukui1 |
| 刊名 | talanta
 |
| 出版日期 | 2015-08-15 |
| 卷号 | 141期号:1页码:235-238 |
| 关键词 | SDS removal On-line digestion Hollow fiber membrane interface Immobilized enzymatic reactor Proteome analysis |
| 通讯作者 | 张丽华 |
| 英文摘要 | in this work, a novel integrated sample preparation device for sds-assisted proteome analysis was developed, by which proteins dissolved in 4% (w/v) sds were first diluted by 50% methanol, and then sds was online removed by a hollow fiber membrane interface (hfmi) with 50 mm ammonium bicarbonate (ph 8.0) as an exchange buffer, finally digested by an immobilized enzyme reactor (imer). to evaluate the performance of such an integrated device, bovine serum albumin dissolved in 4% (w/v) sds as a model sample was analyzed; it could be found that similar to that obtained by direct analysis of bsa digests without sds (the sequence coverage of 60.3 +/- 1.0%, n=3), with hfmi as an interface for sds removal, bsa was identified with the sequence coverage of 61.0 +/- 1.0% (n=3). however, without sds removal by hfmi, bsa could not be digested by the imer and none peptides could be detected. in addition, such an integrated sample preparation device was also applied for the analysis of sds extracted proteins from rat brain, compared to those obtained by filter-aided sample preparation (fasp), not only the identified protein group and unique peptide number were increased by 12% and 39% respectively, but also the sample pretreatment time was shortened from 24 h to 4 h. all these results demonstrated that such an integrated sample preparation device would provide an alternative tool for sds assisted proteome analysis. (c) 2015 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | sample preparation method ; tandem mass-spectrometry ; liquid-chromatography ; gel-electrophoresis ; peptide separation ; identification |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000356987500037 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/143709]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xia, Simin,Yuan, Huiming,Chen, Yuanbo,et al. Integrated SDS removal and protein digestion by hollow fiber membrane based device for SDS-assisted proteome analysis[J]. talanta,2015,141(1):235-238. |
| APA | Xia, Simin,Yuan, Huiming,Chen, Yuanbo,Liang, Zheng,Zhang, Lihua,&Zhang, Yukui.(2015).Integrated SDS removal and protein digestion by hollow fiber membrane based device for SDS-assisted proteome analysis.talanta,141(1),235-238. |
| MLA | Xia, Simin,et al."Integrated SDS removal and protein digestion by hollow fiber membrane based device for SDS-assisted proteome analysis".talanta 141.1(2015):235-238. |
入库方式： OAI收割
来源：大连化学物理研究所
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