A paired ions scoring algorithm based on Morpheus for simultaneous identification and quantification of proteome samples prepared by isobaric peptide termini labeling strategies
文献类型：期刊论文
| 作者 | Zhang, Shen1,2; Wu, Qi1,2; Shan, Yichu1; Sui, Zhigang1; Zhang, Lihua1; Zhang, Yukui1 |
| 刊名 | proteomics
 |
| 出版日期 | 2015-06-01 |
| 卷号 | 15期号:11页码:1781-1788 |
| 关键词 | Bioinformatics Database search algorithm Isobaric peptide termini labeling Morpheus Paired ions scoring algorithm Quantification |
| 通讯作者 | 张丽华 |
| 英文摘要 | the isobaric peptide termini labeling (iptl) method is a promising strategy in quantitative proteomics for its high accuracy, while the increased complexity of ms2 spectra originated from the paired b, y ions has adverse effect on the identification and the coverage of quantification. here, a paired ions scoring algorithm (pisa) based on morpheus, a database searching algorithm specifically designed for high-resolution ms2 spectra, was proposed to address this issue. pisa was first tested on two 1:1 mixed iptl datasets, and increases in peptide to spectrum matchings, distinct peptides and protein groups compared to morpheus itself and mascot were shown. furthermore, the quantification is simultaneously performed and 100% quantification coverage is achieved by pisa since each of the identified peptide to spectrum matchings has several pairs of fragment ions which could be used for quantification. then the pisa was applied to the relative quantification of human hepatocellular carcinoma cell lines with high and low metastatic potentials prepared by an iptl strategy. |
| 学科主题 | 物理化学 |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemical research methods ; biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | tandem mass-spectrometry ; quantitative proteomics ; expression ; databases ; mixtures ; ms/ms ; tags |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000355727400003 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/143710]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Shen,Wu, Qi,Shan, Yichu,et al. A paired ions scoring algorithm based on Morpheus for simultaneous identification and quantification of proteome samples prepared by isobaric peptide termini labeling strategies[J]. proteomics,2015,15(11):1781-1788. |
| APA | Zhang, Shen,Wu, Qi,Shan, Yichu,Sui, Zhigang,Zhang, Lihua,&Zhang, Yukui.(2015).A paired ions scoring algorithm based on Morpheus for simultaneous identification and quantification of proteome samples prepared by isobaric peptide termini labeling strategies.proteomics,15(11),1781-1788. |
| MLA | Zhang, Shen,et al."A paired ions scoring algorithm based on Morpheus for simultaneous identification and quantification of proteome samples prepared by isobaric peptide termini labeling strategies".proteomics 15.11(2015):1781-1788. |
入库方式： OAI收割
来源：大连化学物理研究所
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