Partially isobaric peptide termini labeling assisted proteome quantitation based on MS and MS/MS signals
文献类型：期刊论文
| 作者 | Zhang, Shen1,2; Wu, Qi1,2; Shan, Yichu1; Zhou, Yuan1; Zhang, Lihua1; Zhang, Yukui1 |
| 刊名 | journal of proteomics
 |
| 出版日期 | 2015-01-30 |
| 卷号 | 114期号:1页码:152-160 |
| 关键词 | Quantitative proteomics Partially isobaric peptide termini labeling MS spectra MS/MS spectra Hca-F Hca-P |
| 通讯作者 | 张丽华 |
| 英文摘要 | isotopic labeling and isobaric labeling are two kinds of the typical quantification method that have been widely used in proteomics analysis. herein, a novel quantitative strategy, partially isobaric peptide termini labeling (pitl), was developed to overcome the drawbacks of each above-mentioned labeling strategy, by simultaneously collecting the quantitative information from both ms and ms/ms spectrum. pitl is based on the site-selective n-terminus dimethylation labeling of peptide alpha-n-termini and the free epsilon-amino group of lysines, resulting in the partially isobaric labeling of peptides. the relative quantification can then be achieved by comparing the intensities of precursor ions in ms spectra and a, b and y ions in ms/ms spectra. the quantitative analysis of differently labeled yeast digests pooled with various ratios indicated the good quantitative accuracy, reproducibility, coverage and wide dynamic range of pitl strategy. furthermore, pitl was applied to the quantitative proteome analysis of two mouse hepatocarcinoma ascites syngeneic cell lines with low and high lymph node metastasis rates (hca-f and hca-p). given its low cost, simple operation and good accuracy, pitl might have great potential in the quantitative proteome analysis of biological samples. |
| 学科主题 | 物理化学 |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemical research methods |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | mass-spectrometry ; quantification ; separation ; system ; tags |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000348949100013 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/143712]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R & A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Shen,Wu, Qi,Shan, Yichu,et al. Partially isobaric peptide termini labeling assisted proteome quantitation based on MS and MS/MS signals[J]. journal of proteomics,2015,114(1):152-160. |
| APA | Zhang, Shen,Wu, Qi,Shan, Yichu,Zhou, Yuan,Zhang, Lihua,&Zhang, Yukui.(2015).Partially isobaric peptide termini labeling assisted proteome quantitation based on MS and MS/MS signals.journal of proteomics,114(1),152-160. |
| MLA | Zhang, Shen,et al."Partially isobaric peptide termini labeling assisted proteome quantitation based on MS and MS/MS signals".journal of proteomics 114.1(2015):152-160. |
入库方式： OAI收割
来源：大连化学物理研究所
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