Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation
文献类型：期刊论文
| 作者 | Weng, Yejing1,2; Sui, Zhigang1; Jiang, Hao1,2; Shan, Yichu1; Chen, Lingfan1,2; Zhang, Shen1,2; Zhang, Lihua1; Zhang, Yukui1 |
| 刊名 | scientific reports
 |
| 出版日期 | 2015-04-22 |
| 卷号 | 5期号:1页码:1 |
| 通讯作者 | 张丽华 |
| 英文摘要 | duo to the important roles of n-glycoproteins in various biological processes, the global n-glycoproteome analysis has been paid much attention. however, by current strategies for n-glycoproteome profiling, peptides with glycosylated asn at n-terminus (pgans), generated by protease digestion, could hardly be identified, due to the poor deglycosylation capacity by enzymes. however, theoretically, pgans occupy 10% of n-glycopeptides in the typical tryptic digests. therefore, in this study, we developed a novel strategy to identify pgans by releasing n-glycans through the n-terminal site-selective succinylation assisted enzymatic deglycosylation. the obtained pgans information is beneficial to not only achieve the deep coverage analysis of glycoproteomes, but also discover the new biological functions of such modification. |
| WOS标题词 | science & technology |
| 学科主题 | 物理化学 |
| 类目[WOS] | multidisciplinary sciences |
| 研究领域[WOS] | science & technology - other topics |
| 关键词[WOS] | hydrophilic interaction chromatography ; glycosylation sites ; pngase-f ; glycoproteins ; enrichment ; oligosaccharides ; glycopeptides ; heterogeneity ; proteomics ; identify |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000353283500001 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/143716]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Weng, Yejing,Sui, Zhigang,Jiang, Hao,et al. Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation[J]. scientific reports,2015,5(1):1. |
| APA | Weng, Yejing.,Sui, Zhigang.,Jiang, Hao.,Shan, Yichu.,Chen, Lingfan.,...&Zhang, Yukui.(2015).Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation.scientific reports,5(1),1. |
| MLA | Weng, Yejing,et al."Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation".scientific reports 5.1(2015):1. |
入库方式： OAI收割
来源：大连化学物理研究所
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