Diffusion coefficient measurement by high resolution NMR spectroscopy: Biochemical and pharmaceutical applications
文献类型：期刊论文
| 作者 | Lindon, JC; Liu, ML; Nicholson, JK |
| 刊名 | REVIEWS IN ANALYTICAL CHEMISTRY
 |
| 出版日期 | 1999 |
| 卷号 | 18期号:1-2页码:23-66 |
| 英文摘要 | Recently there has been renewed interest in the measurement of molecular diffusion coefficients using NMR spectroscopy, building an the pioneering experiments of thirty years ago. This has arisen because of the development of new NMR hardware and pulse sequences, which allow the diffusion coefficients relating to individual peaks in a high resolution NMR spectrum to be determined. In our laboratories we have been applying this approach to provide improved characterization of individual molecules in complex mixtures such as biofluids and to measure diffusion coefficients in such mixtures to investigate molecular dynamics and interactions. We have also used the approach to edit NMR spectra of mixtures on the basis of molecular size and thus to characterize monomeric and dimeric species in pharmaceutical materials. We have also been using the measurement of diffusion coefficients to study molecular interactions such as drug-protein binding and the lifetimes of NH protons in peptides and proteins as a measure of their solvent accessibility thus giving information of secondary structure. These recent results are summarized, together with the pertinent experimental details. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | NUCLEAR-MAGNETIC-RESONANCE ; ORDERED 2D NMR ; HUMAN SERUM-ALBUMIN ; HUMAN-BLOOD-PLASMA ; PROTON-EXCHANGE RATES ; FIELD-GRADIENT NMR ; PATTERN-RECOGNITION ; AFFINITY NMR ; SPIN-ECHO ; H-1-NMR SPECTROSCOPY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000080849200003 |
| 公开日期 | 2015-12-01 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/8391]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Univ London Imperial Coll Sci Technol & Med, Div Biomed Sci, London SW7 2AZ, England 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lindon, JC,Liu, ML,Nicholson, JK. Diffusion coefficient measurement by high resolution NMR spectroscopy: Biochemical and pharmaceutical applications[J]. REVIEWS IN ANALYTICAL CHEMISTRY,1999,18(1-2):23-66. |
| APA | Lindon, JC,Liu, ML,&Nicholson, JK.(1999).Diffusion coefficient measurement by high resolution NMR spectroscopy: Biochemical and pharmaceutical applications.REVIEWS IN ANALYTICAL CHEMISTRY,18(1-2),23-66. |
| MLA | Lindon, JC,et al."Diffusion coefficient measurement by high resolution NMR spectroscopy: Biochemical and pharmaceutical applications".REVIEWS IN ANALYTICAL CHEMISTRY 18.1-2(1999):23-66. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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