Determination of the relative NH proton lifetimes of the peptide analogue viomycin in aqueous solution by NMR-based diffusion measurement
文献类型：期刊论文
| 作者 | Liu, ML; Toms, HC; Hawkes, GE; Nicholson, JK; Lindon, JC |
| 刊名 | JOURNAL OF BIOMOLECULAR NMR
 |
| 出版日期 | 1999 |
| 卷号 | 13期号:1页码:25-30 |
| 关键词 | amide diffusion exchange viomycin |
| 英文摘要 | In aqueous solution, exchanging peptide NH protons experience two environments, that of the peptide itself with a relatively slow diffusion coefficient and that of the water solvent with a faster diffusion coefficient. Although in slow exchange on the NMR chemical shift timescale, the magnetic field gradient dependence of the NH peak intensities in an experiment used to measure diffusion coefficients reflects the relative time periods spent in the two environments and this allows the determination of the relative solvent accessibility of exchangeable protons in peptides or proteins. To test this approach, the magnetic field gradient dependent intensities of the chemically shifted amide and amine NH protons of the peptide antibiotic viomycin have been measured using the high resolution longitudinal-eddy-current-delay (LED) NMR method incorporating solvent water peak elimination by non-excitation. The NH resonances of viomycin have been assigned previously and their relative exchange rates determined. Here, the gradient dependence of each NH proton intensity is reported, and these, after a bi-exponential least squares fitting, yield the fractional lifetimes of the protons spent in the peptide and water environments during the diffusion period of the experiment. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Spectroscopy |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Spectroscopy |
| 关键词[WOS] | EXCHANGE RATES ; ORDERED NMR ; CHEMICAL-EXCHANGE ; SPIN-ECHO ; SPECTROSCOPY ; DOSY ; DISTRIBUTIONS ; WATER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000078802400003 |
| 公开日期 | 2015-12-01 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/8397]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Univ London Imperial Coll Sci Technol & Med, Div Biomed Sci, London SW7 2AZ, England 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 3.NW Univ Xian, Instrumental Anal Res Ctr, Xian 710069, Peoples R China 4.Univ London Queen Mary & Westfield Coll, Dept Chem, London E1 4NS, England |
| 推荐引用方式 GB/T 7714 | Liu, ML,Toms, HC,Hawkes, GE,et al. Determination of the relative NH proton lifetimes of the peptide analogue viomycin in aqueous solution by NMR-based diffusion measurement[J]. JOURNAL OF BIOMOLECULAR NMR,1999,13(1):25-30. |
| APA | Liu, ML,Toms, HC,Hawkes, GE,Nicholson, JK,&Lindon, JC.(1999).Determination of the relative NH proton lifetimes of the peptide analogue viomycin in aqueous solution by NMR-based diffusion measurement.JOURNAL OF BIOMOLECULAR NMR,13(1),25-30. |
| MLA | Liu, ML,et al."Determination of the relative NH proton lifetimes of the peptide analogue viomycin in aqueous solution by NMR-based diffusion measurement".JOURNAL OF BIOMOLECULAR NMR 13.1(1999):25-30. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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