Kinetics of the toxic action of Pb2+ on Rhizopus nigricans as studied by microcalorimetry
文献类型：期刊论文
| 作者 | Liu, Y; Yan, CN; Wang, TZ; Zhao, RM; Qu, SS; Shen, P |
| 刊名 | THERMOCHIMICA ACTA
 |
| 出版日期 | 1999-08-03 |
| 卷号 | 333期号:2页码:103-108 |
| 关键词 | microcalorimetry toxicity measurement Pb2+ Rhizopus nigricans thermokinetics |
| 英文摘要 | The microcalorimetric bioassay for acute cellular toxicity is based on metabolic heat production from cultured cells. Microcalorimetry is a quantitative, inexpensive, and versatile method for measuring acute toxicity. The biological response to toxicants is the inhibition of the heat production rate in cells and toxicity is expressed as the concentration of toxicant that is 50% effective in this inhibition (IC50) In this paper, the effect of Pb2+ on Rhizopus nigricans growth was investigated at 25 degrees C. The relationship between growth rate constants (k) and concentration of Pb2+ (C) is k = 0.04226 exp[-3.636 x 10(-5) (C + 177.0)(2)], and IC50 is 47.5 mu g ml(-1). These signals are readily obtained by an LKB 2277-204 heat conduction microcalorimeter. This system can therefore measure acute toxicity for a broad range of toxicants having target sites in various cellular metabolic pathways. (C) 1999 Elsevier Science B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical ; Chemistry, Physical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | MITOCHONDRIAL METABOLISM ; TISSUE ; LIVER |
| 收录类别 | ISTP ; SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000082343900002 |
| 公开日期 | 2015-12-01 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/8398]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China 2.Wuhan Univ, Dept Life Sci, Wuhan 430072, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Y,Yan, CN,Wang, TZ,et al. Kinetics of the toxic action of Pb2+ on Rhizopus nigricans as studied by microcalorimetry[J]. THERMOCHIMICA ACTA,1999,333(2):103-108. |
| APA | Liu, Y,Yan, CN,Wang, TZ,Zhao, RM,Qu, SS,&Shen, P.(1999).Kinetics of the toxic action of Pb2+ on Rhizopus nigricans as studied by microcalorimetry.THERMOCHIMICA ACTA,333(2),103-108. |
| MLA | Liu, Y,et al."Kinetics of the toxic action of Pb2+ on Rhizopus nigricans as studied by microcalorimetry".THERMOCHIMICA ACTA 333.2(1999):103-108. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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