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Chinese Academy of Sciences Institutional Repositories Grid
Kinetics of the toxic action of Pb2+ on Rhizopus nigricans as studied by microcalorimetry

文献类型:期刊论文

作者Liu, Y; Yan, CN; Wang, TZ; Zhao, RM; Qu, SS; Shen, P
刊名THERMOCHIMICA ACTA
出版日期1999-08-03
卷号333期号:2页码:103-108
关键词microcalorimetry toxicity measurement Pb2+ Rhizopus nigricans thermokinetics
英文摘要The microcalorimetric bioassay for acute cellular toxicity is based on metabolic heat production from cultured cells. Microcalorimetry is a quantitative, inexpensive, and versatile method for measuring acute toxicity. The biological response to toxicants is the inhibition of the heat production rate in cells and toxicity is expressed as the concentration of toxicant that is 50% effective in this inhibition (IC50) In this paper, the effect of Pb2+ on Rhizopus nigricans growth was investigated at 25 degrees C. The relationship between growth rate constants (k) and concentration of Pb2+ (C) is k = 0.04226 exp[-3.636 x 10(-5) (C + 177.0)(2)], and IC50 is 47.5 mu g ml(-1). These signals are readily obtained by an LKB 2277-204 heat conduction microcalorimeter. This system can therefore measure acute toxicity for a broad range of toxicants having target sites in various cellular metabolic pathways. (C) 1999 Elsevier Science B.V. All rights reserved.
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical ; Chemistry, Physical
研究领域[WOS]Chemistry
关键词[WOS]MITOCHONDRIAL METABOLISM ; TISSUE ; LIVER
收录类别ISTP ; SCI
语种英语
WOS记录号WOS:000082343900002
公开日期2015-12-01
源URL[http://ir.wipm.ac.cn/handle/112942/8398]  
专题武汉物理与数学研究所_2011年以前论文发表(包括2011年)
作者单位1.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
2.Wuhan Univ, Dept Life Sci, Wuhan 430072, Peoples R China
3.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan, Peoples R China
推荐引用方式
GB/T 7714
Liu, Y,Yan, CN,Wang, TZ,et al. Kinetics of the toxic action of Pb2+ on Rhizopus nigricans as studied by microcalorimetry[J]. THERMOCHIMICA ACTA,1999,333(2):103-108.
APA Liu, Y,Yan, CN,Wang, TZ,Zhao, RM,Qu, SS,&Shen, P.(1999).Kinetics of the toxic action of Pb2+ on Rhizopus nigricans as studied by microcalorimetry.THERMOCHIMICA ACTA,333(2),103-108.
MLA Liu, Y,et al."Kinetics of the toxic action of Pb2+ on Rhizopus nigricans as studied by microcalorimetry".THERMOCHIMICA ACTA 333.2(1999):103-108.

入库方式: OAI收割

来源:武汉物理与数学研究所

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