Assay for Laccase activity by microcalorimetry: Laccase was extracted from China lacquer of Rhus vernicifera
文献类型：期刊论文
| 作者 | Wang, TZ; Li, WP; Wan, HW; Zhou, PJ; Qu, SS |
| 刊名 | JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
 |
| 出版日期 | 2000-08-10 |
| 卷号 | 45期号:1页码:57-63 |
| 关键词 | laccase laccase activity microcalorimetry substrate |
| 英文摘要 | The reactions between Laccase (extracted from China lacquer of Rhus vernicifera) and various substrates (3,4-Dihydroxybenzaldehyde, Guaiacol, Pyrogallol, Gallic acid) have been studied using LKB-2107 batch microcalorimetry system. Based on calorimetry, a new method has been proposed. Laccase activity and the Michaelis constant K-m have been determined simultaneously by this method. The method is simple, sample-saving, and valid for a wider range of substrate concentrations. Furthermore, it can be extended for assaying other enzymes catalyzing reactions using this method. (C) 2000 Elsevier Science B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | INTERMEDIATE ; REDUCTION ; DIOXYGEN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000088647700004 |
| 公开日期 | 2015-12-01 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/8554]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Cent China Normal Univ, Dept Chem, Wuhan 430079, Peoples R China 2.Acad Sinica, Wuhan Inst Phys & Math, Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 3.Wuhan Univ, Coll Chem, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, TZ,Li, WP,Wan, HW,et al. Assay for Laccase activity by microcalorimetry: Laccase was extracted from China lacquer of Rhus vernicifera[J]. JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS,2000,45(1):57-63. |
| APA | Wang, TZ,Li, WP,Wan, HW,Zhou, PJ,&Qu, SS.(2000).Assay for Laccase activity by microcalorimetry: Laccase was extracted from China lacquer of Rhus vernicifera.JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS,45(1),57-63. |
| MLA | Wang, TZ,et al."Assay for Laccase activity by microcalorimetry: Laccase was extracted from China lacquer of Rhus vernicifera".JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS 45.1(2000):57-63. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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