Interaction between calcium-free calmodulin and IQ motif of neurogranin studied by nuclear magnetic resonance spectroscopy
文献类型：期刊论文
| 作者 | Cui, YF; Wen, J; Sze, KH; Man, D; Lin, DH; Liu, ML; Zhu, G |
| 刊名 | ANALYTICAL BIOCHEMISTRY
 |
| 出版日期 | 2003-04-15 |
| 卷号 | 315期号:2页码:175-182 |
| 关键词 | Ca2+-free calmodulin (apoCaM) IQ motif neurogranin dissociation constant (K-d) binding nuclear magnetic resonance (NMR) |
| 英文摘要 | The interaction of Ca2+-free calmodulin (apoCaM) with the IQ motif corresponding to the calmodulin-binding domain of neurogranin has been studied by nuclear magnetic resonance (NMR) methods. The NMR spectra of uncomplexed apoCaM and apoCaM in complex with the IQ motif recorded at 750 MHz were studied and the backbone assignments of the protein in both forms were obtained by triple-resonance multidimensional NMR experiments. Chemical shift perturbations were used to map the binding surfaces. Only a single set of resonances was observed throughout the titration, indicating that the binding interaction is under fast exchange. Analysis of chemical shift changes indicates that (a) the main interaction and conformational changes occur in the C-terminal domain of calmodulin and (b) linker-1 (residues 40-44) between EF-1 and EF-2, linker-3 (residues 112-117) between EF-3 and EF-4, and the end of the alpha-helix H (residues 145-148) may be involved in the binding process. The dissociation constant (K-d), estimated by fitting the chemical shift changes against the IQ peptide concentration, ranged from about 1.2 x 10(-5) to 8.8 x 10(-5) M. This result demonstrates that the interaction falls into the weak binding regime. (C) 2003 Elsevier Science (USA). All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | PROTEIN-KINASE-C ; DIFFUSION MEASUREMENTS ; BINDING ; NMR ; NEUROMODULIN ; SUBSTRATE ; AFFINITY ; DOMAIN ; SYSTEM ; SITE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000182332000005 |
| 公开日期 | 2015-12-08 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/8925]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 2.Hong Kong Univ Sci & Technol, Dept Biochem, Kowloon, Hong Kong, Peoples R China 3.Cent China Normal Univ, Dept Chem, Wuhan 430079, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, YF,Wen, J,Sze, KH,et al. Interaction between calcium-free calmodulin and IQ motif of neurogranin studied by nuclear magnetic resonance spectroscopy[J]. ANALYTICAL BIOCHEMISTRY,2003,315(2):175-182. |
| APA | Cui, YF.,Wen, J.,Sze, KH.,Man, D.,Lin, DH.,...&Zhu, G.(2003).Interaction between calcium-free calmodulin and IQ motif of neurogranin studied by nuclear magnetic resonance spectroscopy.ANALYTICAL BIOCHEMISTRY,315(2),175-182. |
| MLA | Cui, YF,et al."Interaction between calcium-free calmodulin and IQ motif of neurogranin studied by nuclear magnetic resonance spectroscopy".ANALYTICAL BIOCHEMISTRY 315.2(2003):175-182. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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