Analysis of competitive binding of ligands to human serum albumin using NMR relaxation measurements
文献类型：期刊论文
| 作者 | Cui, YF; Bai, GY; Li, CG; Ye, CH; Liu, ML |
| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
 |
| 出版日期 | 2004-02-04 |
| 卷号 | 34期号:2页码:247-254 |
| 关键词 | NMR spectroscopy H-1 relaxation rate competitive binding ibuprofen salicylic acid human serum albumin |
| 英文摘要 | The competitive binding of two ligands, ibuprofen (IBP) and salicylic acid (SAL), to human serum albumin (HSA) was studied by using nuclear magnetic resonance (NMR) relaxation measurements. When the concentration of one ligand was increased in the solution containing IBP, SAL and HSA, the fractions of free IBP and SAL were increased because of the competitive binding. The H-1 relaxation rates (R-1) of both ligands were subsequently decreased. If a ligand is in fast exchanging between the free and bound forms, the observed H-1 relaxation rate is a weighted average of that for the free ligand and the protein-ligand complex. The concentrations of the free and bound ligands can be quantitatively derived from the relaxation rates. The results presented in this work revealed that IBP and SAL shared certain low-affinity binding sites on the HSA molecule, in addition to the same high-affinity binding site of AIII. (C) 2003 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine |
| 类目[WOS] | Chemistry, Analytical ; Pharmacology & Pharmacy |
| 研究领域[WOS] | Chemistry ; Pharmacology & Pharmacy |
| 关键词[WOS] | LOW-AFFINITY BINDING ; DIFFUSION MEASUREMENTS ; PROTEIN-BINDING ; IBUPROFEN ; ENANTIOMERS ; DRUGS ; SITE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000189331400001 |
| 公开日期 | 2015-12-08 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/8958]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 2.Cent China Normal Univ, Dept Chem, Wuhan 430079, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, YF,Bai, GY,Li, CG,et al. Analysis of competitive binding of ligands to human serum albumin using NMR relaxation measurements[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2004,34(2):247-254. |
| APA | Cui, YF,Bai, GY,Li, CG,Ye, CH,&Liu, ML.(2004).Analysis of competitive binding of ligands to human serum albumin using NMR relaxation measurements.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,34(2),247-254. |
| MLA | Cui, YF,et al."Analysis of competitive binding of ligands to human serum albumin using NMR relaxation measurements".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 34.2(2004):247-254. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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