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Influence of pH value on the conformation transition of silk fibroin

文献类型：期刊论文

作者Xie, X; Zhou, P; Deng, F; Wu, PY; Zong, XH; Yao, WH
刊名CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
出版日期2004-05-01
卷号25期号:5页码:961-965
关键词silk fibroin conformation transition influence of PH value NMR Raman spectroscopy
英文摘要Both C-13 CP-MAS solid state NMR and laser Raman scattering spectroscopy were used to investigate the influence of pH values on the conformation transition of silk fibroin of Bambyx mori silkworm. The regenerated silk fibroins in the phosphate buffer solution were used to imitate the silk fibroins of the gland. A series of membranes formed in buffer solutions with different pH values were obtained by the evaporation of water at room temperature(for mimicking the gradual loss of water in spinning process). The composes and conformation in the fibroin membranes were determined qualitatively by Raman and Generalized Two-Dimensional Correlation Raman Spectroscopy for amide I region(1 600-1 700 cm(-1)) of the silk fibroins. There are four types of conformations at least existing in the fibroin membranes, including conformations of typical silk I (a-form and/or random coil), typical silk I (beta-sheet), silk I transition and silk I transition, etc.. Based on the qualitative results from 2D Raman spectrum 1 the relationship between pH value and conformation contents in the studied systems was determined quantitatively by simulation of C-beta peak (delta 14. 5-22) in C-13 CP-MAS solid state NMR spectrum. The results show that acidic solution (pH = 5.2) is more favorable than neutral (pH = 6.9) and basic (pH = 8.0) ones for the conformation transition from Silk I to Silk I because the silk fibroin molecular backbones are easier to extend and then to form beta-sheets in acidic solution.
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]SOLID-STATE ; NMR-SPECTROSCOPY ; CHEMICAL-SHIFTS ; RAMAN ; MECHANISM ; IONS
收录类别SCI
语种英语
WOS记录号WOS:000221514700044
公开日期2015-12-08
源URL[http://ir.wipm.ac.cn/handle/112942/8976]  
专题武汉物理与数学研究所_2011年以前论文发表（包括2011年）
作者单位1.Fudan Univ, Dept Macromol Sci, Minist Educ, Key Lab Mol Engn, Shanghai 200433, Peoples R China
2.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China
3.Fudan Univ, Analyt Measurement Ctr, Shanghai 200433, Peoples R China
推荐引用方式
GB/T 7714		 Xie, X,Zhou, P,Deng, F,et al. Influence of pH value on the conformation transition of silk fibroin[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2004,25(5):961-965.
APA Xie, X,Zhou, P,Deng, F,Wu, PY,Zong, XH,&Yao, WH.(2004).Influence of pH value on the conformation transition of silk fibroin.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,25(5),961-965.
MLA Xie, X,et al."Influence of pH value on the conformation transition of silk fibroin".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 25.5(2004):961-965.

入库方式： OAI收割

来源：武汉物理与数学研究所

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