Diversity and distribution of autotrophic microbial community along environmental gradients in grassland soils on the Tibetan Plateau
文献类型：SCI/SSCI论文
| 作者 | Guo G. X.; Kong, W. D.; Liu, J. B.; Zhao, J. X.; Du, H. D.; Zhang, X. Z.; Xia, P. H. |
| 发表日期 | 2015 |
| 关键词 | RubisCO CO2 fixation Tibetan Plateau Grassland Elevation large-subunit genes covered antarctic lake altitudinal gradient bacterial communities agricultural soils elevation gradient alpine grasslands species richness organic-matter carbon-dioxide |
| 英文摘要 | Soil microbial autotrophs play a significant role in CO2 fixation in terrestrial ecosystem, particularly in vegetation-constrained ecosystems with environmental stresses, such as the Tibetan Plateau characterized by low temperature and high UV. However, soil microbial autotrophic communities and their driving factors remain less appreciated. We investigated the structure and shift of microbial autotrophic communities and their driving factors along an elevation gradient (4400-5100 m above sea level) in alpine grassland soils on the Tibetan Plateau. The autotrophic microbial communities were characterized by quantitative PCR, terminal restriction fragment length polymorphism (T-RFLP), and cloning/sequencing of cbbL genes, encoding the large subunit for the CO2 fixation protein ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO). High cbbL gene abundance and high RubisCO enzyme activity were observed and both significantly increased with increasing elevations. Path analysis identified that soil RubisCO enzyme causally originated from microbial autotrophs, and its activity was indirectly driven by soil water content, temperature, and NH4 (+) content. Soil autotrophic microbial community structure dramatically shifted along the elevation and was jointly driven by soil temperature, water content, nutrients, and plant types. The autotrophic microbial communities were dominated by bacterial autotrophs, which were affiliated with Rhizobiales, Burkholderiales, and Actinomycetales. These autotrophs have been well documented to degrade organic matters; thus, metabolic versatility could be a key strategy for microbial autotrophs to survive in the harsh environments. Our results demonstrated high abundance of microbial autotrophs and high CO2 fixation potential in alpine grassland soils and provided a novel model to identify dominant drivers of soil microbial communities and their ecological functions. |
| 出处 | Applied Microbiology and Biotechnology |
| 卷 | 99 |
| 期 | 20 |
| 页 | 8765-8776 |
| 收录类别 | SCI |
| 语种 | 英语 |
| ISSN号 | 0175-7598 |
| 源URL | [http://ir.igsnrr.ac.cn/handle/311030/38628]  |
| 专题 | 地理科学与资源研究所_历年回溯文献 |
| 推荐引用方式 GB/T 7714 | Guo G. X.,Kong, W. D.,Liu, J. B.,et al. Diversity and distribution of autotrophic microbial community along environmental gradients in grassland soils on the Tibetan Plateau. 2015. |
入库方式： OAI收割
来源：地理科学与资源研究所
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