The Role of Glutathione Detoxification Pathway in MCLR-Induced Hepatotoxicity in SD Rats
文献类型：期刊论文
| 作者 | Li, Shangchun1,2; Chen, Jun1; Xie, Ping1; Guo, Xiaochun1,3; Fan, Huihui1,3; Yu, Dezhao1,2; Zeng, Cheng1,3; Chen, Liang1,2 |
| 刊名 | ENVIRONMENTAL TOXICOLOGY
 |
| 出版日期 | 2015-12-01 |
| 卷号 | 30期号:12页码:1470-1480 |
| 关键词 | microcystin-LR glutathione oxidative stress liver BSO SD rat |
| ISSN号 | 1520-4081 |
| 英文摘要 | In the present study, we investigated the role of glutathione (GSH) and its related enzymes in Sprague Dawley (SD) rats subjected to microcystin-leucine-arginine (MCLR)-induced hepatotoxicity. SD rats were intraperitoneally (i.p.) injected with MCLR after pretreating with or without buthionine-(S,R)-sulfoximine (BSO), an inhibitor of GSH synthesis. The depletion of GSH with BSO enhanced MCLR-induced oxidative stress, resulting in more severe liver damage and higher MCLR accumulation. Similarly, the contents of malondialdehyde (MDA), total GSH (T-GSH), oxidized GSH (GSSG) and GSH were significantly enhanced in BSO pretreated rats following MCLR treatment. The study showed that the transcription of GSH-related enzymes such as glutathione-S-transferase (GST), gamma-glutamylcysteine synthetase (gamma-GCS), glutathione reductase (GR) varied in different ways (expect for glutathione peroxidase (GPx), whose gene expression was induced in all treated groups) with or without BSO pretreatment before MCLR exposure, suggesting an adaptative response of GSH-related enzymes at transcription level to combat enhancement of oxidative stress induced by MCLR when pretreated with BSO. These data suggested the tissues with low GSH concentration are highly vulnerable to MCLR toxicity and GSH was critical for the detoxification in MCLR-induced hepatotoxicity in vivo. (C) 2014 Wiley Periodicals, Inc. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Environmental Sciences ; Toxicology ; Water Resources |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Toxicology ; Water Resources |
| 关键词[WOS] | INDUCED DNA-DAMAGE ; MICROCYSTIN-LR ; OXIDATIVE STRESS ; CYANOBACTERIAL TOXINS ; ANTIOXIDANT ENZYMES ; HUMAN HEALTH ; IN-VIVO ; LIVER ; TOXICITY ; EXPOSURE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000364781900012 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/27356]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Shangchun,Chen, Jun,Xie, Ping,et al. The Role of Glutathione Detoxification Pathway in MCLR-Induced Hepatotoxicity in SD Rats[J]. ENVIRONMENTAL TOXICOLOGY,2015,30(12):1470-1480. |
| APA | Li, Shangchun.,Chen, Jun.,Xie, Ping.,Guo, Xiaochun.,Fan, Huihui.,...&Chen, Liang.(2015).The Role of Glutathione Detoxification Pathway in MCLR-Induced Hepatotoxicity in SD Rats.ENVIRONMENTAL TOXICOLOGY,30(12),1470-1480. |
| MLA | Li, Shangchun,et al."The Role of Glutathione Detoxification Pathway in MCLR-Induced Hepatotoxicity in SD Rats".ENVIRONMENTAL TOXICOLOGY 30.12(2015):1470-1480. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。