Evaluation the effect of thiamin deficiency on intestinal immunity of young grass carp (Ctenopharyngodon idella)
文献类型：期刊论文
| 作者 | Wen, Ling-Mei1; Jiang, Wei-Dan1,2; Liu, Yang1,2; Wu, Pei1,2; Zhao, Juan1; Jiang, Jun1,2; Kuang, Sheng-Yao4; Tang, Ling4; Tang, Wu-Neng4; Zhang, Yong-An5 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2015-10-01 |
| 卷号 | 46期号:2页码:501-515 |
| 关键词 | Thiamin deficiency Grass carp Immune response Tight junction Antioxidant capacity |
| ISSN号 | 1050-4648 |
| 英文摘要 | Our study explored the effect of dietary thiamin on growth and immunity (intestine, head kidney, spleen and liver) of young grass carp (Ctenopharyngodon idella). Fish were fed diets containing six graded levels of thiamin at 0.12-2.04 mg/kg diet for 8 weeks. The percentage weight gain (PWG), feed intake and feed efficiency were lower in fish fed the 0.12 mg/kg diet. Thiamin deficiency decreased complement 3 content, lysozyme (LA) and acid phosphatase activities, mRNA levels of hepcidin and interleukin (IL) 10, elevated mRNA levels of interferon gamma 2, tumor necrosis factor alpha, IL-10 and IL-8 in intestine, head kidney, spleen and liver. The mRNA levels of inhibitor protein-kappa B alpha, target of rapamycin (TOR) and NF-E2-related factor 2 (Nrf2), the activities and mRNA levels of copper/zinc superoxide dismutase, manganese superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and glutathione reductase were down-regulated, mRNA levels of myosin light-chain kinase (MLCK), I kappa B kinases (IKK beta and IKK gamma), nuclear factor kappa B P65 (NF-kappa B P65) and Kelch-like-ECH-associated protein la (Keap1a) were up-regulated in the intestine of fish fed the thiamin-deficient diet. Additionally, thiamin deficiency decreased claudin b, c and 3, ZO-1 and occludin mRNA levels in each intestinal segment, increased claudin 12 and claudin 15a mRNA levels in distal intestine. In conclusion, thiamin deficiency decreased fish growth and immunity of intestine, head kidney, spleen and liver. The dietary thiamin requirement of young grass carp (242-742 g) based on intestinal LA activity or PWG were determined to be 1.15 or 0.90 mg/kg diet, respectively. (C) 2015 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | NF-KAPPA-B ; SALMON SALMO-SALAR ; CYPRINUS-CARPIO ; GROWTH-PERFORMANCE ; MUCOSAL IMMUNE ; TRANSCRIPT ABUNDANCE ; DISEASE-RESISTANCE ; ANTIOXIDANT STATUS ; DIETARY THIAMIN ; GENE-EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000363071200039 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/27334]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Peoples R China 2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Peoples R China 3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Peoples R China 4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wen, Ling-Mei,Jiang, Wei-Dan,Liu, Yang,et al. Evaluation the effect of thiamin deficiency on intestinal immunity of young grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2015,46(2):501-515. |
| APA | Wen, Ling-Mei.,Jiang, Wei-Dan.,Liu, Yang.,Wu, Pei.,Zhao, Juan.,...&Feng, Lin.(2015).Evaluation the effect of thiamin deficiency on intestinal immunity of young grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,46(2),501-515. |
| MLA | Wen, Ling-Mei,et al."Evaluation the effect of thiamin deficiency on intestinal immunity of young grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 46.2(2015):501-515. |
入库方式： OAI收割
来源：水生生物研究所
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