The tight junction protein transcript abundance changes and oxidative damage by tryptophan deficiency or excess are related to the modulation of the signalling molecules, NF-kappa B p65, TOR, caspase-(3,8,9) and Nrf2 mRNA levels, in the gill of young grass carp (Ctenopharyngodon idellus)
文献类型：期刊论文
| 作者 | Jiang, Wei-Dan1,2,3; Wen, Hai-Lang1; Liu, Yang1,2,3; Jiang, Jun1; Kuang, Sheng-Yao4; Wu, Pei1,2,3; Zhao, Juan1; Tang, Ling4; Tang, Wu-Neng4; Zhang, Yong-An5 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2015-10-01 |
| 卷号 | 46期号:2页码:168-180 |
| 关键词 | Tight junction protein Cytokine Apoptosis Antioxidant defense Tryptophan Gene expression |
| ISSN号 | 1050-4648 |
| 英文摘要 | This study is for the first time to explore the possible effects of dietary tryptophan (Trp) on structural integrity and the related signalling factor gene expression in the gill of young grass carp (Ctenopharyngodon idella). Fish were fed with six different experimental diets containing graded levels of Trp at 0.7 (control), 1.7, 3.1, 4.0, 5.2 and 6.1 g kg(-1) diet for 8 weeks. The results firstly demonstrated that Trp deficiency or excess caused increases in reactive oxygen species (ROS) contents, and severe oxidative damage (lipid peroxidation and protein oxidation) in the gill of fish, and those negative effects could be reversed by optimal Trp levels. Secondly, compared with the optimal Trp levels, Trp deficiency could cause decreases in the mRNA levels of the barrier functional proteins (occludin, zonula occludens-1, claudin-c, and -3) and increases in the mRNA levels of the pore-formation proteins (claudin-12 and -15) mRNA levels in the gill of fish, and those were reversed by the optimal levels of Trp. The negative effects of Trp deficiency on those tight junction protein gene expression might be partly related to the increases in the mRNA levels of pro-inflammatory cytokines and related signalling factors (tumor necrosis factor a, interleukin 8, interleukin 10 and transcription factor-kappa B) and decreases in the mRNA levels of anti-inflammatory cytokines and related signalling factors [interleukin 10, transforming growth factor-beta 1, nuclear inhibitor factor kappa B alpha (i kappa B alpha), target of rapamyc and ribosome protein S6 kinase 1 (S6K1)] in the gill of fish. In addition, optimal dietary Trp protected the gill of fish against its deficiency-caused increases in the mRNA levels of the apoptosis signalling (caspase-3, caspase-8, caspase-9) and decreases in anti-superoxide radicals capacity, anti-hydroxyl radical capacity, glutathione contents and the activities of Cu/Zn superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST) in the gill of fish. Additionally, compared with the Trp deficiency, optimal Trp up-regulated the mRNA levels of SOD, CAT, GPx, GR and GST, which might be partly ascribed to the up-regulation of the NF-E2-related factor 2 (Nrf2) mRNA levels and the down-regulation of Kelch-like-ECH-associated protein 1 (Keap1) mRNA levels in the gill of fish. Interestingly, excessive Trp caused similar results with its deficiency. Collectively, Trp deficiency or excess could cause antioxidant system disruption and change tight junction protein transcription abundances, which were partly related to the signalling factors, NF-kappa B p65, TOR, caspase-(3,8,9) and Nrf2, in fish gill, those could be blocked by the optimal Trp levels. (C) 2015 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | PROGRAMMED CELL-DEATH ; ERYTHROID 2-RELATED FACTOR-2 ; NUCLEAR FACTOR-KAPPAB ; GENE-EXPRESSION ; ANTIOXIDANT DEFENSES ; ENZYME-ACTIVITIES ; AMINO-ACID ; IMMUNE-RESPONSES ; BARRIER FUNCTION ; COPPER EXPOSURE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000363071200002 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/27336]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China 2.Sichuan Agr Univ, Fish Nutr & Safety Prod Sichuan Univ Key Lab, Chengdu 611130, Sichuan, Peoples R China 3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China 4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Wei-Dan,Wen, Hai-Lang,Liu, Yang,et al. The tight junction protein transcript abundance changes and oxidative damage by tryptophan deficiency or excess are related to the modulation of the signalling molecules, NF-kappa B p65, TOR, caspase-(3,8,9) and Nrf2 mRNA levels, in the gill of young grass carp (Ctenopharyngodon idellus)[J]. FISH & SHELLFISH IMMUNOLOGY,2015,46(2):168-180. |
| APA | Jiang, Wei-Dan.,Wen, Hai-Lang.,Liu, Yang.,Jiang, Jun.,Kuang, Sheng-Yao.,...&Feng, Lin.(2015).The tight junction protein transcript abundance changes and oxidative damage by tryptophan deficiency or excess are related to the modulation of the signalling molecules, NF-kappa B p65, TOR, caspase-(3,8,9) and Nrf2 mRNA levels, in the gill of young grass carp (Ctenopharyngodon idellus).FISH & SHELLFISH IMMUNOLOGY,46(2),168-180. |
| MLA | Jiang, Wei-Dan,et al."The tight junction protein transcript abundance changes and oxidative damage by tryptophan deficiency or excess are related to the modulation of the signalling molecules, NF-kappa B p65, TOR, caspase-(3,8,9) and Nrf2 mRNA levels, in the gill of young grass carp (Ctenopharyngodon idellus)".FISH & SHELLFISH IMMUNOLOGY 46.2(2015):168-180. |
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来源：水生生物研究所
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