The integration characteristics of the exogenous growth hormone gene in a transgenic common carp (Cyprinus carpio L.) with fast-growth performance
文献类型：期刊论文
| 作者 | Chen, Ji1; Luo, Qing1,2; Bao, Hairong1; Liao, Lanjie1; Li, Yongming1; Zhu, Zuoyan1; Wang, Yaping1; Hu, Wei1 |
| 刊名 | SCIENCE BULLETIN
 |
| 出版日期 | 2015-10-01 |
| 卷号 | 60期号:19页码:1654-1660 |
| 关键词 | Common carp Growth hormone Transgene Integration site Copy number |
| ISSN号 | 2095-9273 |
| 英文摘要 | The genetic stability and expression efficiency of exogenous genes in transgenic animals are closely related to integration site and copy number. In our laboratory, by transgenic manipulation and subsequent test crosses, we established an "all-fish" growth hormone (GH) transgenic common carp family that exhibits fast growth. In this present study, genome walking, real-time quantitative polymerase chain reaction, and fluorescence in situ hybridization techniques were applied to identify the integration characteristics of the exogenous grass carp GH gene in the transgenic common carp. The exogenous GH genes, in the form of two complete and one incomplete tandem repeats, were found to have integrated into an AT-rich region near the end of a chromosome pair. We hypothesize that the high efficiency of exogenous GH gene expression might be due to the low copy number in the genome and the AT-rich integration site. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | SALMON ONCORHYNCHUS-KISUTCH ; MISGURNUS-MIZOLEPIS ; ACCELERATED-GROWTH ; ZEBRAFISH EMBRYOS ; ATLANTIC SALMON ; MUD LOACH ; FISH ; EXPRESSION ; GENOME ; CELLS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000363043800004 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/27332]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Ji,Luo, Qing,Bao, Hairong,et al. The integration characteristics of the exogenous growth hormone gene in a transgenic common carp (Cyprinus carpio L.) with fast-growth performance[J]. SCIENCE BULLETIN,2015,60(19):1654-1660. |
| APA | Chen, Ji.,Luo, Qing.,Bao, Hairong.,Liao, Lanjie.,Li, Yongming.,...&Hu, Wei.(2015).The integration characteristics of the exogenous growth hormone gene in a transgenic common carp (Cyprinus carpio L.) with fast-growth performance.SCIENCE BULLETIN,60(19),1654-1660. |
| MLA | Chen, Ji,et al."The integration characteristics of the exogenous growth hormone gene in a transgenic common carp (Cyprinus carpio L.) with fast-growth performance".SCIENCE BULLETIN 60.19(2015):1654-1660. |
入库方式： OAI收割
来源：水生生物研究所
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