Gene Deletion Strategy To Examine the Involvement of the Two Chondroitin Lyases in Flavobacterium columnare Virulence
文献类型：期刊论文
| 作者 | Li, Nan1; Qin, Ting1; Zhang, Xiao Lin1; Huang, Bei2; Liu, Zhi Xin1; Xie, Hai Xia1; Zhang, Jin1; McBride, Mark J.3; Nie, Pin1 |
| 刊名 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
 |
| 出版日期 | 2015-11-01 |
| 卷号 | 81期号:21页码:7394-7402 |
| ISSN号 | 0099-2240 |
| 英文摘要 | Flavobacterium columnare is an important bacterial pathogen of freshwater fish that causes high mortality of infected fish and heavy economic losses in aquaculture. The pathogenesis of this bacterium is poorly understood, in part due to the lack of efficient methods for genetic manipulation. In this study, a gene deletion strategy was developed and used to determine the relationship between the production of chondroitin lyases and virulence. The F. johnsoniae ompA promoter (PompA) was fused to sacB to construct a counterselectable marker for F. columnare. F. columnare carrying PompA-sacB failed to grow on media containing 10% sucrose. A suicide vector carrying PompA-sacB was constructed, and a gene deletion strategy was developed. Using this approach, the chondroitin lyase-encoding genes, cslA and cslB, were deleted. The Delta cslA and Delta cslB mutants were both partially deficient in digestion of chondroitin sulfate A, whereas a double mutant (Delta cslA Delta cslB) was completely deficient in chondroitin lyase activity. Cells of F. columnare wild-type strain G(4) and of the chondroitin lyase-deficient Delta cslA Delta cslB mutant exhibited similar levels of virulence toward grass carp in single-strain infections. Coinfections, however, revealed a competitive advantage for the wild type over the chondroitin lyase mutant. The results indicate that chondroitin lyases are not essential virulence factors of F. columnare but may contribute to the ability of the pathogen to compete and cause disease in natural infections. The gene deletion method developed in this study may be employed to investigate the virulence factors of this bacterium and may have wide application in many other members of the phylum Bacteroidetes. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 关键词[WOS] | AC LYASE ; GLIDING MOTILITY ; CYTOPHAGA JOHNSONAE ; FISH ; G(4) ; CONSTRUCTION ; MUTAGENESIS ; INSERTIONS ; CLONING ; GENOME |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000363462900006 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/27340]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei Province, Peoples R China 2.Jimei Univ, Coll Fisheries, Xiamen, Fujian Province, Peoples R China 3.Univ Wisconsin, Dept Biol Sci, Milwaukee, WI 53201 USA |
| 推荐引用方式 GB/T 7714 | Li, Nan,Qin, Ting,Zhang, Xiao Lin,et al. Gene Deletion Strategy To Examine the Involvement of the Two Chondroitin Lyases in Flavobacterium columnare Virulence[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2015,81(21):7394-7402. |
| APA | Li, Nan.,Qin, Ting.,Zhang, Xiao Lin.,Huang, Bei.,Liu, Zhi Xin.,...&Nie, Pin.(2015).Gene Deletion Strategy To Examine the Involvement of the Two Chondroitin Lyases in Flavobacterium columnare Virulence.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,81(21),7394-7402. |
| MLA | Li, Nan,et al."Gene Deletion Strategy To Examine the Involvement of the Two Chondroitin Lyases in Flavobacterium columnare Virulence".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 81.21(2015):7394-7402. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。