Molecular characterization and expression of an oocyte-specific histone stem-loop binding protein in Carassius gibelio
文献类型：期刊论文
| 作者 | Liu, Zhen; Zhang, Xiao-Juan; Wang, Wei; Zhang, Jun; Li, Zhi; Gui, Jian-Fang |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
 |
| 出版日期 | 2015-12-01 |
| 卷号 | 190页码:46-53 |
| 关键词 | Carassius gibelio Stem-loop-binding protein SLBP2 Oocyte Oogenesis |
| ISSN号 | 1096-4959 |
| 英文摘要 | Stem-loop-binding proteins (SLBPs) have been revealed to interact with stem-loop of histones, and oocyte-specific and oocyte-preferential SLBP2 have been identified in vertebrates including Xenopus (S. tropicalis) and B. taurus to play a key role in histone translation regulation, but no oocyte-specific SLBPs have been characterized in fish. Here, we have identified and characterized the first fish oocyte-specific SLBP2 in Carassius gibelio. Its full-length cDNA contains 975 bp ORF encoding 324 amino acids. Firstly, the polyclonal antibody specific to C gibelio SLBP2 was prepared. Then, RT-PCR analysis and Western blot detection revealed its oocyte-specific and dynamic expression pattern during oogenesis and embryogenesis of C gibelio. Moreover, in situ hybridization and immunofluorescence localization observed its abundant expression in cortical alveolar stage oocytes and dynamic distribution in different stage oocytes. Altogether, our current data suggest that C. gibelio SLBP2 might play significant role in the early oogenesis and oocyte growth. (C) 2015 Elsevier Inc All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Zoology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Zoology |
| 关键词[WOS] | SILVER CRUCIAN CARP ; PRE-MESSENGER-RNA ; AURATUS-GIBELIO ; CORTICAL GRANULES ; GENETIC-BASIS ; ZEBRAFISH ; FISH ; TRANSITION ; MATURATION ; OOGENESIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000364252600006 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/27347]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | Univ Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Zhen,Zhang, Xiao-Juan,Wang, Wei,et al. Molecular characterization and expression of an oocyte-specific histone stem-loop binding protein in Carassius gibelio[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2015,190:46-53. |
| APA | Liu, Zhen,Zhang, Xiao-Juan,Wang, Wei,Zhang, Jun,Li, Zhi,&Gui, Jian-Fang.(2015).Molecular characterization and expression of an oocyte-specific histone stem-loop binding protein in Carassius gibelio.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,190,46-53. |
| MLA | Liu, Zhen,et al."Molecular characterization and expression of an oocyte-specific histone stem-loop binding protein in Carassius gibelio".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 190(2015):46-53. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。