Specific Cleavage of the Nucleoprotein of Fish Rhabdovirus
文献类型：期刊论文
| 作者 | Zhou, G. -Z.1; Yi, Y. -J.1; Chen, Z. -Y.2; Zhang, Q. -Y.2 |
| 刊名 | VETERINARY PATHOLOGY
 |
| 出版日期 | 2015-11-01 |
| 卷号 | 52期号:6页码:1258-1262 |
| 关键词 | mandarin fish rhabdovirus nucleoprotein cleavage caspases virology apoptosis |
| ISSN号 | 0300-9858 |
| 英文摘要 | Siniperca chuatsi rhabdovirus (SCRV) is one of myriad rhabdoviruses recorded in fish. Preliminary data show that inhibition of the SCRV nucleoprotein (N) could significantly reduce the progeny virus titers in infected Epithelioma papulosum cyprinid (EPC) cells. Here, the authors propose that cleavage of the viral 47-kDa N protein is caspase-mediated based on caspase inhibition experiments, transient expression in EPC transfection, and analysis of cleavage sites. Cleavage of the SCRV N protein in culture was prevented by a pan-caspase inhibitor, z-VAD-FMK (z-Val-Ala-DL-Asp-fluoromethyl ketone). Subsequently, N was transiently expressed in EPC cells, the results of which indicated that the specific cleavage of N also occurred in the cells transfected with N-GFP plasmid. Several truncated fragments of the N gene were constructed and transiently transfected into EPC cells. Immunoblotting results indicated that D324 and D374 are the cleavage sites of N by caspases. The authors also found that z-VAD-FMK could inhibit the cytopathic effect in SCRV-infected EPC cells but not affect the production of infectious progeny, suggesting that the caspase-mediated cleavage of N protein is not required for in vitro SCRV replication. To the authors' knowledge, this is the first report on the cleavage of rhabdovirus proteins. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Pathology ; Veterinary Sciences |
| 研究领域[WOS] | Pathology ; Veterinary Sciences |
| 关键词[WOS] | VIRUS-INFECTION ; CELL-LINE ; APOPTOSIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000364734300039 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/27354]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Henan Univ Technol, Coll Bioengn, Zhengzhou 450001, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, G. -Z.,Yi, Y. -J.,Chen, Z. -Y.,et al. Specific Cleavage of the Nucleoprotein of Fish Rhabdovirus[J]. VETERINARY PATHOLOGY,2015,52(6):1258-1262. |
| APA | Zhou, G. -Z.,Yi, Y. -J.,Chen, Z. -Y.,&Zhang, Q. -Y..(2015).Specific Cleavage of the Nucleoprotein of Fish Rhabdovirus.VETERINARY PATHOLOGY,52(6),1258-1262. |
| MLA | Zhou, G. -Z.,et al."Specific Cleavage of the Nucleoprotein of Fish Rhabdovirus".VETERINARY PATHOLOGY 52.6(2015):1258-1262. |
入库方式： OAI收割
来源：水生生物研究所
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