Lysozyme as a recognition element for monitoring of bacterial population
文献类型：期刊论文
| 作者 | Zheng, Laibao1 ; Wan, Yi2; Yu, Liangmin1; Zhang, Dun2
|
| 刊名 | TALANTA
 |
| 出版日期 | 2016 |
| 卷号 | 146期号:2015页码:299-302 |
| 关键词 | Lysozyme Recognition element Bacterial population Bacterial detection |
| 英文摘要 | Bacterial infections remain a significant challenge in biomedicine and environment safety. Increasing worldwide demand for point-of-care techniques and increasing concern on their safe development and use, require a simple and sensitive bioanalysis for pathogen detection. However, this goal is not yet achieved. A design for fluorescein isothiocyanate-labeled lysozyme (FITC-LYZ), which provides quantitative binding information for gram-positive bacteria, Micrococcus luteus, and detects pathogen concentration, is presented. The functional lysozyme is used not only as the pathogenic detection platform, but also as a tracking reagent for microbial population in antibacterial tests. A nonlinear relationship between the system response and the logarithm of the bacterial concentration was observed in the range of 1.2 x 10(2)-1.2 x 10(5) cfu mL(-1). The system has a potential for further applications and provides a facile and simple method for detection of pathogenic bacteria. Meanwhile, the fluorescein isothiocyanate-labeled lysozyme is also employed as the tracking agent for antibacterial dynamic assay, which show a similar dynamic curve compared with UV-vis test. (C) 2015 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000363815600042 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/82288]  |
| 专题 | 海洋研究所_海洋腐蚀与防护研究发展中心 |
| 作者单位 | 1.Ocean Univ China, Minist Educ, Key Lab Marine Chem Theory & Technol, Qingdao 266100, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Laibao,Wan, Yi,Yu, Liangmin,et al. Lysozyme as a recognition element for monitoring of bacterial population[J]. TALANTA,2016,146(2015):299-302. |
| APA | Zheng, Laibao,Wan, Yi,Yu, Liangmin,&Zhang, Dun.(2016).Lysozyme as a recognition element for monitoring of bacterial population.TALANTA,146(2015),299-302. |
| MLA | Zheng, Laibao,et al."Lysozyme as a recognition element for monitoring of bacterial population".TALANTA 146.2015(2016):299-302. |
入库方式： OAI收割
来源：海洋研究所
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