中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Early transcriptional response to the parasitic dinoflagellate Hematodinium in hepatopancreas of Portunus trituberculatus

文献类型:期刊论文

作者Li, Meng1,2; Wang, Jinfeng1,2; Song, Shuqun1; Li, Caiwen1
刊名JOURNAL OF INVERTEBRATE PATHOLOGY
出版日期2015-09-01
卷号130期号:2015页码:28-36
关键词Crustacean Parasite Histopathology Enzyme activity Transcription Innate immunity
英文摘要The swimming crab Portunus trituberculatus supports a large proportion of crab aquaculture in China. In the last decade, the sustainable culture of this crab was threatened by the parasitic dinoflagellate Hematodinuim, resulting in massive mortality (up to 95%) in severely impacted culture ponds. Previous studies of Hematodinium were mainly focused on histology, molecular characterization, epizootiology, etc., with limited studies conducted to explore this specific host-parasite interaction. Thus, to give a primary insight into the anti-parasitic immune response at the critical stage of infection, the expression levels of 8 immune-related genes together with enzyme activities of phenoloxidase (PO), alkaline phosphatase (AMP), and acid phosphatase (ACP), were evaluated in hepatopancreas during 3-192 h (h) post inoculation. Hematoxylin-eosin (H&E) staining showed noticeable pathological changes in hepatopancreas. The enzyme activities of PO, AKP, and ACP were significantly induced after inoculation. The changes of the prophenoloxidase (proPO) transcripts and the constantly enhanced PO activity reflected the critical function of the proPO system in resisting against the parasites. The decreased expression levels of LGBP and PPAF implied an immunosuppressive mechanism of the parasites against the host proPO system. And the significant variations in transcriptional levels of two important proteinase inhibitors (serpin, alpha 2m) and three P. trituberculatus clip-domain serine proteinases (PTcSPs) suggested that the parasites could affect proteinase cascade reactions associated with immune response by destroying the balance between serine proteinases and the inhibitors. Moreover, the results indicated that the hepatopancreas of P. trituberculatus was significantly affected by invasion of the parasite, and hepatopancreas played important roles in the crustacean innate immunity against the parasitic infection. (C) 2015 Elsevier Inc. All rights reserved.
收录类别SCI
语种英语
WOS记录号WOS:000361258800005
源URL[http://ir.qdio.ac.cn/handle/337002/50879]  
专题海洋研究所_海洋生态与环境科学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Li, Meng,Wang, Jinfeng,Song, Shuqun,et al. Early transcriptional response to the parasitic dinoflagellate Hematodinium in hepatopancreas of Portunus trituberculatus[J]. JOURNAL OF INVERTEBRATE PATHOLOGY,2015,130(2015):28-36.
APA Li, Meng,Wang, Jinfeng,Song, Shuqun,&Li, Caiwen.(2015).Early transcriptional response to the parasitic dinoflagellate Hematodinium in hepatopancreas of Portunus trituberculatus.JOURNAL OF INVERTEBRATE PATHOLOGY,130(2015),28-36.
MLA Li, Meng,et al."Early transcriptional response to the parasitic dinoflagellate Hematodinium in hepatopancreas of Portunus trituberculatus".JOURNAL OF INVERTEBRATE PATHOLOGY 130.2015(2015):28-36.

入库方式: OAI收割

来源:海洋研究所

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