Development of a fluorescence in situ hybridization (FISH) method for rapid detection of Ulva prolifera
文献类型：期刊论文
| 作者 | Zhang, Qing-Chun1; Liu, Qing1,2 ; Kang, Zhen-Jun1,2; Yu, Ren-Cheng1; Yan, Tian1; Zhou, Ming-Jiang1
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| 刊名 | ESTUARINE COASTAL AND SHELF SCIENCE
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| 出版日期 | 2015-09-20 |
| 卷号 | 163期号:2015页码:103-111 |
| 关键词 | fluorescence in situ hybridization (FISH) Ulva prolifera green tide Yellow Sea |
| 英文摘要 | Large-scale green tides have occurred consecutively since 2007 in the Yellow Sea (YS), China. The dominant causative species of the green tides has been identified as Ulva prolifera. The origin of green tides in the YS has been traced back to the Subei Shoal based on the results of remote-sensing, numerical simulations and field investigations. However, it is difficult to study the early development of green tides in the Subei Shoal because of the mixture of multiple green algae and the morphological diversity of U. prolifera when under variable environmental conditions. In this study, a rapid and accurate fluorescence in situ hybridization (FISH) method was developed to detect U. prolifera from the community of green algae targeting the 5S rDNA spacer region of U. prolifera. Two specific probes, 5S-1 and 5S-2, were designed based on the sequences of the 5S rDNA spacer regions of U. prolifera, Ulva linza and Ulva flexuosa. Specificity of the FISH method was tested using the six species of green algae commonly occurring in the Subei Shoal, including U. prolifera, U. linza, U. flexuosa, Ulva compressa, Ulva pertusa and Blidingia sp. The results showed that only U. prolifera could be labeled with both probes. Probe 5S-1, which showed a much higher labeling efficiency on U. prolifera, was ultimately selected as the probe for the FISH detection. The sample preparation method was optimized, particularly for the mature green algae, by the addition of cellulase and proteinase K in the pre-hybridization solution. Labeling efficiency with the probe 5S-1 reached 96% on average under the optimized conditions. The successful development of the FISH method has been applied to qualitative and quantitative analysis of field samples collected from the YS, and the results indicate a potential use in future green algae studies. (C) 2014 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000362604700017 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/52135]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Qing-Chun,Liu, Qing,Kang, Zhen-Jun,et al. Development of a fluorescence in situ hybridization (FISH) method for rapid detection of Ulva prolifera[J]. ESTUARINE COASTAL AND SHELF SCIENCE,2015,163(2015):103-111. |
| APA | Zhang, Qing-Chun,Liu, Qing,Kang, Zhen-Jun,Yu, Ren-Cheng,Yan, Tian,&Zhou, Ming-Jiang.(2015).Development of a fluorescence in situ hybridization (FISH) method for rapid detection of Ulva prolifera.ESTUARINE COASTAL AND SHELF SCIENCE,163(2015),103-111. |
| MLA | Zhang, Qing-Chun,et al."Development of a fluorescence in situ hybridization (FISH) method for rapid detection of Ulva prolifera".ESTUARINE COASTAL AND SHELF SCIENCE 163.2015(2015):103-111. |
入库方式： OAI收割
来源：海洋研究所
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